Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers
Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers
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DOI：
10.1002/anie.200702919
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发表时间：
2007-01-01
影响因子：
16.6
通讯作者：
Nishimura, Shin-ichiro
中科院分区：
文献类型：
--
作者：
Kurogochi, Masaki;Amano, Maho;Nishimura, Shin-ichiro
糖肽的鉴定：采用新型的反糖印迹技术，建立了一种高通量的糖蛋白组学方法。该方法包括对糖肽末端唾液酸残基进行高选择性氧化，然后用聚合物试剂进行化学连接富集（见示意图，Neu5Ac: n -乙酰神经氨酸；Neu5Gc: n -糖基神经氨酸；绿色：n -乙酰- d -葡萄糖胺；黄色：d -甘露糖；蓝色：d -半乳糖）。
(Chemical Equation Presented) Identification of glycopeptides: A high-throughput glycoproteomics approach was established by using novel reverse-glycoblotting technology. The method involves highly selective oxidation of the terminal sialic acid residues of glycopeptides and subsequent enrichment by chemical ligation with a polymer reagent (see schematic representation; Neu5Ac: N-acetylneuraminic acid; Neu5Gc: N-glycolylneuraminic acid; green: N-acetyl-D-glucosamine; yellow: D-mannose; blue: D-galactose).