Growth and surface properties of new thermoacidophilic Archaea strain Acidianus manzaensis YN-25 grown on different substrates
Growth and surface properties of new thermoacidophilic Archaea strain Acidianus manzaensis YN-25 grown on different substrates
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DOI：
10.1016/s1003-6326(09)60011-x
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发表时间：
2008-12-01
影响因子：
4.5
通讯作者：
Nie Zhen-yuan
中科院分区：
文献类型：
--
作者：
He Huan;Yang Yi;Nie Zhen-yuan
研究了从云南腾冲酸性温泉中分离到的嗜热嗜酸菌Acidianus manzaensis YN-25在可溶性基质硫酸亚铁和不溶性固体基质S-0、黄铁矿和黄铜矿上的生长和表面性质。通过改变细胞数量、pH、E、Fe 2+浓度、SO 42-浓度、[Fe 2 +]/[Fe 3 +]比值等表征细胞在各基质中的生长特性，并通过Zeta电位、疏水性和细胞表面FT-IR光谱表征和分析细胞的表面性质。结果表明，在固体基质上生长的细胞具有较高的等电点。它们比硫酸亚铁生长的细胞更疏水，表达更多的表面蛋白。
The growth and surface properties of new thermoacidophilic Archaea strain Acidianus manzaensis YN-25 isolated from an acid hot spring in Tengchong, Yunnan Province, China were investigated cultured on different substrates including soluble substrate ferrous sulfate and nonsoluble solid substrates S-0, pyrite and chalcopyrite. The growth characteristics of the cells in each substrate were characterized with the changes in cell number, pH, E, and concentrations of Fe2+ or SO42-, or ratios of [Fe2+] to [Fe3+], and the surface properties were characterized and analyzed in terms of Zeta-potential, hydrophobicity, and surface FT-IR spectra of the cells. The results show that the cells grown on solid substrates have higher value of isoelectric points. They are more hydrophobic and express more surface proteins than ferrous sulfate grown cells.