Reproductive Influences of Erythromycin and Sulfamethoxazole on Caenorhabditis elegans over Generations Mediated by Lipid Metabolism
Reproductive Influences of Erythromycin and Sulfamethoxazole on Caenorhabditis elegans over Generations Mediated by Lipid Metabolism
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DOI：
10.1007/s40242-023-3024-2
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发表时间：
2023-05
影响因子：
3.1
通讯作者：
Zhenyang Yu;Wen Wang;Gaotian Li;Jing Zhang
中科院分区：
文献类型：
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作者：
Zhenyang Yu;Wen Wang;Gaotian Li;Jing Zhang
抗生素是具有伪持久性的新兴污染物。然而，很少探讨其世代生殖毒性和潜在机制。本文研究了红霉素（ERY）和磺胺甲恶唑（SMZ）对秀丽隐杆线虫（C. habditis elegans）的生殖影响。elegans）的F_1 ~ F_4代多代暴露，以及它们对F_1和F_4代非直接暴露后代（T_4和T_4 ′）的影响，探讨其跨代效应。在高浓度下，ERY和SMZ均对生殖产生抑制作用，且随着世代数的增加，抑制作用逐渐减弱。在低浓度下，SMZ刺激T4中的繁殖，与其在F1中的抑制相反。进一步对F1 ~ F4代脂肪和糖脂代谢中的10种酶和蛋白质的分析表明，ERY对F1 ~ F4代脂肪酸合成酶（FAS）具有普遍的抑制作用，ERY和SMZ对其他生化物质的作用表现为既有促进作用，又有抑制作用。综合生物标志物响应指数（IBR）值表明，ERY和SMZ对F1代的总体代谢有促进作用，但在后代中这种促进作用减弱甚至转变为抑制作用。在比较多代和跨代效应时，高浓度的ERY和SMZ对T4代的生殖有抑制作用，对T4 '代有促进作用，而对F1和F4代则相反。
Antibiotics are emerging pollutants with pseudo-persistence. However, their reproductive toxicities over generations and underlying mechanisms were seldom explored. In the present study, reproductive effects of erythromycin(ERY) and sulfamethoxazole(SMZ) were studied onCaenorhabditis elegans(C. elegans) with multi-generational exposure from F1 to F4, and their effects in the non-direct exposed offspring of F1 and F4(T4 and T4’) were explored to investigate trans-generational outcomes. At high concentrations, ERY and SMZ inhibited the reproduction and the inhibition decreased with more generations’ exposure. At low concentrations, SMZ stimulated reproduction in T4, opposite to its inhibition in F1. Further analysis on ten enzymes and proteins in lipid and glucolipid metabolism showed that ERY commonly inhibited fatty acid synthetase(FAS) in F1 to F4, while ERY and SMZ showed stimulation and inhibition on other biochemicals. Integrated biomarker response(IBR) index values showed that ERY and SMZ stimulated the overall metabolism in F1, and the stimulation decreased and even altered to inhibition in later generations. When comparing multi- and trans-generational effects, ERY and SMZ at high concentration caused inhibition on reproduction in T4 and stimulation in T4’, which were opposite to those in F1 and F4.