Comprehensive assay of flavanones in citrus juices and beverages by UHPLC-ESI-MS/MS and derivatization chemistry
Comprehensive assay of flavanones in citrus juices and beverages by UHPLC-ESI-MS/MS and derivatization chemistry
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DOI：
10.1016/j.foodchem.2013.05.034
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发表时间：
2013-12-01
期刊：
影响因子：
8.8
通讯作者：
Sindona, Giovanni
中科院分区：
文献类型：
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作者：
Di Donna, Leonardo;Taverna, Domenico;Sindona, Giovanni
采用液相色谱-串联质谱（LC-MS/MS）和衍生化学法，以d（0）/d（3）甲氧基胺为内标物，对水果和蔬菜中大量存在的黄酮类化合物黄烷酮进行了分析。该测定方法考虑了13种黄烷酮，包括苷元、新橙皮苷、芸香苷和3-羟基-3-甲基戊二酰衍生物。该方法的优势在于相对较短的分析时间（16 min）和良好的重复性和再现性值，在大多数情况下低于10%（RSD%）。准确度值范围为95.4%-111.3%，而LOQ值范围为0.05 - 0.29 mg/L，具体取决于分析物。(C)2013爱思唯尔有限公司保留所有权利。
Flavanones, a class of flavonoids present in large amounts in fruits and vegetables, have been assayed by LC-MS/MS and derivatization chemistry using d(0)/d(3)-labelled derivatized internal standards obtained by simple reaction procedures which involves d(0)/d(3) methoxyamine. The assay method considers 13 flavanones including aglycones, neohesperidosides, rutinosides and 3-hydroxy-3-methyl glutaryl derivatives. The strengths of the method consist in a relative short analysis time (16 min) and good repeatability and reproducibility values which are in most cases under 10% (RSD%). The accuracy values range from 95.4% to 111.3% whilst the LOQ values ranges from 0.05 to 0.29 mg/L depending on the analyte. (C) 2013 Elsevier Ltd. All rights reserved.