EFFECTS OF PROLACTIN ON ALPHA-CHLORIDE AND BETA-CHLORIDE CELLS IN THE GILL EPITHELIUM OF THE SALTWATER ADAPTED TILAPIA OREOCHROMIS-NILOTICUS
EFFECTS OF PROLACTIN ON ALPHA-CHLORIDE AND BETA-CHLORIDE CELLS IN THE GILL EPITHELIUM OF THE SALTWATER ADAPTED TILAPIA OREOCHROMIS-NILOTICUS
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DOI：
10.1002/ar.1092350211
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发表时间：
1993-02-01
影响因子：
--
通讯作者：
RAMBOURG, A
中科院分区：
文献类型：
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作者：
PISAM, M;AUPERIN, B;RAMBOURG, A
罗非鱼（Oreochromis niloticus）平均体重21 g，分为两组。一组在淡水中维持，而另一组在千分之二十的盐水中适应2周。在后者中，每2天注射罗非鱼催乳素（ti-PRL I），持续一周。制备鳃片用于电镜观察，以确定在每种实验条件下氯细胞的类型和表面积。根据淡水鱼鳃中的位置和超微结构特征，很容易区分出α和β两种类型的氯细胞，而在适应咸水的动物中只遇到一种类型的细胞，即可能由淡水α细胞转化而来的咸水α细胞。对适应咸水的鱼类注射PRL后，在鳃片间区域重新出现了与淡水罗非鱼β细胞超微结构特征相似的小氯细胞。在相同的实验条件下，大量的盐水α细胞显示出恢复淡水α细胞超微结构特征的趋势，而淡水α细胞是它们的来源。这些观察结果表明，催乳素是一种“淡水适应激素”，而β细胞在鱼类适应淡水生活条件中起着特殊的作用。
Tilapia (Oreochromis niloticus), 21 g average body weight, were divided into two groups. A group was maintained in fresh water, whereas another group was adapted for 2 weeks to 20 parts per thousand salt water. Among the latter, fishes were injected every 2 days for a week with tilapia prolactin (ti-PRL I). Gills were prepared for electron microscopy in order to determine the types and surface areas of chloride cells in each experimental condition. Two types of chloride cells, the alpha and beta cells were easily distinguished on the basis of their location and ultrastructural features in the gills of freshwater fishes, while only one type of cell, the saltwater alpha cells presumably derived from the transformation of the freshwater alpha cells, were encountered in saltwater adapted animals. After PRL injection of saltwater adapted fishes, small chloride cells, which displayed ultrastructural features similar to those of beta cells in freshwater tilapia, reappeared in interlamellar regions of the gills. In the same experimental conditions, the voluminous saltwater alpha cells showed a tendency to resume ultrastructural features more characteristic of the freshwater a cells from which they were derived. These observations tend to indicate that prolactin behaves as a ''freshwater adapting hormone'' and that beta cells are specifically involved in fish adaptation to freshwater living conditions.