Relative timing of redistribution of cytochrome c and Smac/DIABLO from mitochondria during apoptosis assessed by double immunocytochemistry on mammalian cells
Relative timing of redistribution of cytochrome c and Smac/DIABLO from mitochondria during apoptosis assessed by double immunocytochemistry on mammalian cells
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DOI：
10.1016/j.yexcr.2006.01.026
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发表时间：
2006-04-15
影响因子：
3.7
通讯作者：
Nagley, P
中科院分区：
文献类型：
--
作者：
Lim, MLR;Chen, BH;Nagley, P
细胞色素c和Smac/DIABLO从线粒体的重新分配发生在细胞凋亡过程中，虽然它们的释放的相对时间没有得到很好的表征。本文采用免疫细胞化学双标法定量研究了单细胞线粒体释放细胞色素c和Smac/DIABLO的模式。人骨肉瘤细胞和小鼠胚胎皮层神经元在星形孢菌素诱导的凋亡过程中进行了分析。在用星形孢菌素处理24小时的骨肉瘤细胞中，相当大比例的细胞（36%）在Smac/DIABLO之前从线粒体释放细胞色素c。相比之下，这些蛋白质在神经元中大部分是一致释放的;只有少数细胞（
Redistribution of cytochrome c and Smac/DIABLO from mitochondria occurs during apoptosis, although the relative timing of their release is not well characterized. Double immunocytochemistry was utilized here to study quantitatively the patterns of release of cytochrome c and Smac/DIABLO from mitochondria in single cells. Human osteosarcoma cells and murine embryonic cortical neurons were analyzed during apoptosis induced by staurosporine. In osteosarcoma cells treated with staurosporine for 24 h, a substantial proportion of cells (36%) released cytochrome c from the mitochondria before Smac/DIABLO. In contrast, these proteins were released mostly concordantly in neurons; only a minority of cells (