Endosomal recycling regulates Anthrax Toxin Receptor 1/Tumor Endothelial Marker 8-dependent cell spreading.
Endosomal recycling regulates Anthrax Toxin Receptor 1/Tumor Endothelial Marker 8-dependent cell spreading.
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DOI：
10.1016/j.yexcr.2010.03.026
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发表时间：
2010-07-15
影响因子：
3.7
通讯作者：
Werner E
中科院分区：
文献类型：
--
作者：
Gu J;Faundez V;Werner E
Mechanisms for receptor-mediated anthrax toxin internalization and delivery to the cytosol are well understood. However, far less is known about the fate followed by anthrax toxin receptors prior and after cell exposure to the toxin. We report that Anthrax Toxin Receptor 1/Tumor Endothelial Marker 8 (TEM8) localized at steady state in Rab11a-positive and transferrin receptor-containing recycling endosomes. TEM8 followed a slow constitutive recycling route of ∼30 minutes as determined by pulsed surface biotinylation and chase experiments. A Rab11a dominant negative mutant and Myosin Vb tail expression impaired TEM8 recycling by sequestering TEM8 in intracellular compartments. Sequestration of TEM8 in intracellular compartments with monensin coincided with increased TEM8 association with a multi-protein complex isolated with antibodies against transferrin receptor. Addition of the cell-binding component of anthrax toxin, Protective Antigen, reduced TEM8 half-life from seven to three hours, without preventing receptor recycling. Pharmacological and molecular perturbation of recycling endosome function using monensin, dominant negative Rab11a, or myosin Vb tail, reduced PA-binding efficiency and TEM8-dependent cell spreading on PA-coated surfaces without affecting toxin delivery to the cytosol. These results indicate that the intracellular fate of TEM8 differentially affect its cell adhesion and cell intoxication functions.
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影响因子：
4.8
作者：
Gage, RM;Kim, KA;von Zastrow, M
通讯作者：
von Zastrow, M
影响因子：
64.5
作者：
BASU, SK;GOLDSTEIN, JL;BROWN, MS
通讯作者：
BROWN, MS
DOI：
10.1073/pnas.79.10.3162
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LEPPLA, SH
通讯作者：
LEPPLA, SH
影响因子：
3.3
作者：
Lapierre, LA;Kumar, R;Goldenring, JR
通讯作者：
Goldenring, JR
影响因子：
7.8
作者：
Abrami, Laurence;Leppla, Stephen H;van der Goot, F Gisou
通讯作者：
van der Goot, F Gisou