Ethylene preparation and its application to physiological experiments
Ethylene preparation and its application to physiological experiments
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DOI：
10.4161/psb.5.4.10875
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发表时间：
2010-04
影响因子：
2.9
通讯作者：
Wei Zhang;Wenli Hu;C. Wen
中科院分区：
文献类型：
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作者：
Wei Zhang;Wenli Hu;C. Wen
乙烯是第一个被发现的调节植物生长发育多方面的气体激素。ACC和Ethephon是两种广泛使用的化学品，在没有乙烯可用时替代乙烯处理。然而，ACC和Ethephon转化的乙烯量是不可控制的，这使得对于对乙烯浓度、响应窗口和处理时间敏感的实验来说，这两种处理是否能模拟乙烯的影响是值得怀疑的。乙烯可以通过乙醇脱水来化学合成，但需要从脱水产物中进一步提纯。我们以前报道过，在缓冲条件下分解乙烯利可以很容易地制备乙烯气体，生成的乙烯可以直接使用。乙烯反应可以通过测量黄化幼苗的下胚轴长度或通过ERF1(乙烯反应因子1)的表达来估计。虽然低浓度的ACC不足以诱导ERF1的表达，但在乙烯处理不可行的实验中，高浓度的ACC可以替代乙烯。然而，ACC可能会经历早期消费。开发了多种方法，使缺乏乙烯和气体处理技术的实验室可以轻松地进行必要的乙烯处理。
Ethylene is the first identified gaseous hormone regulating many aspects of plant growth and development. ACC and ethephon are two widely used chemicals replacing ethylene treatment when ethylene is not available. However, the amount of ethylene converted by ACC and ethephon is not controllable, leaving it questionable whether either treatment can mimic the effects of ethylene for experiments that are sensitive to ethylene concentration, response window, and treatment durations. Ethylene can be chemically made by ethanol dehydration; however, further purification from the dehydration products is needed. We previously reported that the ethylene gas can be easily prepared by decomposing ethephon in a buffered condition and the resulting ethylene can be used directly. Ethylene responses can be estimated by the measurement of the hypocotyl length of etiolated seedlings, or by ERF1 (Ethylene Response Factor1) expression. Although ACC of low concentrations is insufficient to induce ERF1 expression, ACC of high concentrations can replace ethylene for experiments where ethylene treatment is not feasible. However, ACC may undergo early consumption. Versatile approaches were developed so that laboratories lacking ethylene and techniques for gas handling can easily perform necessary ethylene treatments.