Advanced targeting strategies for murine retroviral and adeno-associated viral vectors.
Advanced targeting strategies for murine retroviral and adeno-associated viral vectors.
复制标题
鼠逆转录病毒和腺相关病毒载体的高级靶向策略。
DOI：
10.1007/10_006
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Schaffer,DavidV
中科院分区：
文献类型：
--
作者：
Yu,JulieH;Schaffer,DavidV
Targeted gene delivery involves broadening viral tropism to infect previously nonpermissive cells, replacing viral tropism to infect a target cell exclusively, or stealthing the vector against nonspecific interactions with host cells and proteins. These approaches offer the potential advantages of enhanced therapeutic effects, reduced side effects, lowered dosages, and enhanced therapeutic economics. This review will discuss a variety of targeting strategies, both genetic and nongenetic, for re-engineering the tropism of two representative enveloped and nonenveloped viruses, murine retrovirus and adeno-associated virus. Basic advances in understanding the structural biology and virology of the parent viruses have aided rational design efforts to engineer novel properties into the viral attachment proteins. Furthermore, even in the absence of basic, mechanistic knowledge of viral function, high-throughput library and directed evolution approaches can yield significant improvements in vector function. These two complementary strategies offer the potential to gain enhanced molecular control over vector properties and overcome challenges in generating high titer, stealthy, retargeted vectors.
