Characterization of the inflammatory and apoptotic cells in the aortas of patients with ascending thoracic aortic aneurysms and dissections
Characterization of the inflammatory and apoptotic cells in the aortas of patients with ascending thoracic aortic aneurysms and dissections
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DOI：
10.1016/j.jtcvs.2005.09.018
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发表时间：
2006-03-01
影响因子：
6
通讯作者：
Milewicz, DM
中科院分区：
文献类型：
--
作者：
He, RM;Guo, DC;Milewicz, DM
目的：升主动脉瘤和夹层的组织病理学异常是中膜变性，这种病变被描述为平滑肌细胞和弹性纤维的非炎性损失。本研究试图确定是否存在炎症细胞中膜变性和评估任何可能的贡献，这些细胞的平滑肌细胞凋亡。主动脉标本取自接受升主动脉瘤（n = 9）和A型夹层（n = 7）预防性手术修复的患者，沿着对照组患者死于与主动脉疾病无关的原因（n = 5）。免疫组化染色进行评估的淋巴细胞和巨噬细胞的存在下，细胞凋亡的标志物进行了评估，在升主动脉瘤和dispos.Results患者的动脉瘤和dispositions的CD3（+）细胞的免疫组化研究表明，显着更多的CD3（+）细胞在动脉瘤或夹层患者的动脉瘤比对照动脉瘤（P = 0.020和P = 0.0022，分别）。此外，动脉瘤或夹层患者的动脉瘤有更多的CD68（+）细胞（分别为P = 0.01和P = 0.005）。CD3（+）细胞分布于中膜及外膜血管周围。在原位末端转移酶介导的脱氧尿苷三磷酸缺口末端标记中，相对于对照组动脉瘤或夹层患者的动脉瘤中，发现细胞数量增加（分别为P = 0.005和P = 0.002）。此外，相对于对照动脉瘤，动脉瘤和夹层患者的主动脉样本中Fas和FasL增加。在动脉瘤和A型夹层的升支动脉中，炎性细胞与凋亡性血管细胞死亡的标志物共存，这增加了活化的T细胞和巨噬细胞可能有助于平滑肌细胞的消除和与动脉瘤相关的基质的降解的可能性。胸主动脉瘤和夹层。
Objective: The histopathologic abnormality underlying ascending aortic aneurysm and dissection is medial degeneration, a lesion that is described as the noninflammatory loss of smooth muscle cells and elastic fibers. This study sought to determine whether inflammatory cells are present in medial degeneration and assess any possible contribution of these cells to apoptosis of smooth muscle cells.Methods: Aortic specimens were obtained from patients undergoing prophylactic surgical repair of an ascending aortic aneurysm ( n = 9) and type A dissection ( n = 7), along with control patients dying of causes unrelated to aortic disease ( n = 5). Immunohistochemical staining was performed to evaluate the presence of lymphocytes and macrophages, and markers of apoptosis were assessed in the aortas of patients with ascending aortic aneurysm and dissection.Results: Immunohistochemical study indicated significantly more CD3(+) cells in the aortas of patients with aneurysms or dissections than in control aortas ( P = .020 and P = .0022, respectively). In addition, aortas of patients with aneurysms or dissections had more CD68(+) cells ( P = .01 and P = .005, respectively). CD3(+) cells were localized in the media and surrounding the vasa vasorum in the adventitia. Cells yielding a positive result on in situ terminal transferase - mediated deoxyuridine triphosphate nick end- labeling were found in increased numbers in the aortas of patients with aneurysms or dissections relative to control aortas ( P = .005 and P = .002, respectively). Furthermore, Fas and FasL were increased in the aortic samples from patients with aneurysms and dissections relative to control aortas.Conclusion: The coexistence of inflammatory cells with markers of apoptotic vascular cell death in the media of ascending aortas with aneurysms and type A dissections raises the possibility that activated T cells and macrophages may contribute to the elimination of smooth muscle cells and degradation of the matrix associated with thoracic aortic aneurysms and dissections.