Collagen absorption bands in heated and rehydrated dentine
Collagen absorption bands in heated and rehydrated dentine
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DOI：
10.1016/j.saa.2005.03.025
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发表时间：
2005-12-01
影响因子：
4.4
通讯作者：
Zezell, DM
中科院分区：
文献类型：
--
作者：
Bachmann, L;Gomes, ASL;Zezell, DM
这项工作的目的是确定在加热和再水合的牙本质胶原带的变化。我们使用的牛牙质切片在烤箱中加热，温度在100到300摄氏度之间。样品水化在氯化钠溶液中进行，浓度为0.9 wt.%；傅里叶变换红外光谱仪在4000 ~ 400 cm（-1）的光谱范围内采集光谱。我们的研究结果表明，在温度范围内（t225℃），胶原蛋白变性，补液后没有观察到还原。这项工作确定了牙本质胶原蛋白在组织加热和再水化时可以呈现的一个重要特征；这些结果表明牙本质胶原蛋白的变性温度接近175-200摄氏度(c) 2005 Elsevier B.V.版权所有。
The objective of this work is identifying changes in the collagen bands in heated and rehydrated dentine. We use bovine dentine slices that were heated in oven between 100 and 300 degrees C. The sample hydration was conducted in sodium chloride solution at 0.9 wt.%; the spectra were acquired by a Fourier transform infrared spectrometer in the spectral range of 4000-400 cm(-1). Our results show a temperature range (T 225 degrees C) where the collagen is denatured and no reversion is observed after rehydration. This work identifies an important characteristic that dentinal collagen can assume when the tissue is heated and rehydrated; these results indicate the denaturation temperature of dentinal collagen to be near 175-200 degrees C. (c) 2005 Elsevier B.V. All rights reserved.