Physiological and immunocytochemical evidence for a putative H-K-ATPase in elasmobranch renal acid secretion.
Physiological and immunocytochemical evidence for a putative H-K-ATPase in elasmobranch renal acid secretion.
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软骨鱼肾酸分泌中推定的 H-K-ATP 酶的生理和免疫细胞化学证据。
DOI：
10.1152/ajprenal.1994.267.4.f639
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Smolka,AJ
中科院分区：
文献类型：
--
作者：
Swenson,ER;Fine,AD;Maren,TH;Reale,E;Lacy,ER;Smolka,AJ
海洋鱼类肾脏酸分泌的机制在很大程度上是未知的。本研究探讨了H（+）-K（+）-腺苷三磷酸酶（H（+）-K（+）-ATP酶）在板鳃角鲨肾脏中是否存在及其在酸分泌中的功能。在整个动物研究中，哺乳动物H（+）-K（+）-ATP酶的特异性抑制剂Sch-28080消除了超过87%的基础（62 mg/kg）和75%的咪唑刺激的可滴定酸排泄（5和62 mg/kg）。针对COOH末端猪胃H（+）-K（+）-ATP酶α亚单位的抗体染色这些鱼的颈部，早期和晚期近端小管，晚期中间小管，远端小管的两个部分，和早期集合管的肾小管的特定细分。这些发现与一种类似于哺乳动物胃H（+）-K（+）-ATP酶的蛋白质在板鳃类动物肾脏酸分泌中的主要作用一致。
The mechanism of renal acid secretion in marine fish is largely unknown. We explored whether H(+)-K(+)-adenosinetriphosphatase (H(+)-K(+)-ATPase) is present and functional in acid secretion in the kidney of the elasmobranch spiny dogfish shark, Squalus acanthias. In whole animal studies, a specific inhibitor of mammalian H(+)-K(+)-ATPase, Sch-28080, abolished greater than 87% of basal (62 mg/kg) and 75% of imidazole-stimulated titratable acid excretion (5 and 62 mg/kg). Antibodies directed against the COOH-terminus hog gastric H(+)-K(+)-ATPase alpha-subunit stained specific subdivisions of the neck, early and late proximal tubule, late intermediate tubule, both segments of the distal tubule, and the early collecting duct of the renal tubule of these fish. These findings are consistent with a major role for a protein similar to the mammalian gastric H(+)-K(+)-ATPase in elasmobranch renal acid secretion.