L-Phenylalanine supplementation increases the production of phenylalanine ammonia-lyase and methyl cinnamate in the mycelia of Tricholoma matsutake
L-Phenylalanine supplementation increases the production of phenylalanine ammonia-lyase and methyl cinnamate in the mycelia of Tricholoma matsutake
复制标题
补充 L-苯丙氨酸可增加松茸菌丝体中苯丙氨酸解氨酶和肉桂酸甲酯的产量
DOI：
10.1016/j.myc.2017.07.001
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发表时间：
2018
期刊：
影响因子：
1.4
通讯作者：
Miho Kato
中科院分区：
文献类型：
--
作者：
Yuji Tasaki;Shunya Hayashi;Miho Kato
研究了松茸口蘑NBRC 30605菌丝生长过程中编码苯丙氨酸氨分析酶（pal）的Tmpal1和Tmpal2的表达变化，以及外源添加pal底物l -苯丙氨酸（L-Phe）对pal表达和肉桂酸甲酯生成的影响。Tmpal1和Tmpal2在菌丝生长过程中的表达模式不同。在添加4e6 mM L-Phe的培养中，Tmpal2转录物水平、PAL活性和肉桂酸甲酯含量显著增加。这些结果表明，补充L-Phe增加了主要负责Tmpal2表达的PAL活性；因此，肉桂酸甲酯由l -苯丙氨酸大量产生。
We investigated changes in the expression of Tmpal1 and Tmpal2, that encode phenylalanine ammonialyases (PALs), during the growth of Tricholoma matsutake NBRC 30605 mycelia, and the effect of exogenously added L-phenylalanine (L-Phe), a substrate of PALs, on pal expression and methyl cinnamate production in the mycelia. Expression patterns of Tmpal1 and Tmpal2 differed during mycelial growth. Transcript levels of Tmpal2, PAL activity, and methyl cinnamate content increased markedly in cultures supplemented with 4e6 mM L-Phe. These results indicate that L-Phe supplementation increases PAL activity that is mainly responsible for Tmpal2 expression; consequently, methyl cinnamate is abundantly produced from L-Phe.