Further evidence that ribavirin interacts with eIF4E
Further evidence that ribavirin interacts with eIF4E
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DOI：
10.1261/rna.2238705
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发表时间：
2005-12-01
期刊：
影响因子：
4.5
通讯作者：
Borden, KLB
中科院分区：
文献类型：
--
作者：
Kentsis, A;Volpon, L;Borden, KLB
这篇评论讨论了Yan及其同事和Westman及其同事最近在RNA中关于利巴韦林在体外与重组eIF 4 E结合并抑制细胞提取物的7-甲基鸟苷帽依赖性外源mRNA翻译的明显失败的报道。测量结合使用亲和层析的基质固定化蛋白质，并通过使用蛋白质发射荧光光谱在核苷酸配体的存在下，以及使用细胞提取物的局限性进行了讨论的mRNA翻译的机制的评估。Yan及其同事和Westman及其同事的不一致结果的可能原因被提出，并通过使用质谱法直接观察利巴韦林与eIF 4 E的特异性结合。
This commentary discusses the recent reports in RNA by Yan and colleagues and Westman and colleagues of the apparent failure of ribavirin to bind to recombinant eIF4E and inhibit 7-methyl guanosine cap-dependent exogenous mRNA translation of cell extracts in vitro. Measuring binding by using affinity chromatography of matrix-immobilized proteins and by using protein emission fluorescence spectroscopy in the presence of nucleotide ligands, as well as limitations of using cell extracts for the assessment of mechanisms of mRNA translation are discussed. Possible reasons for the discordant findings of Yan and colleagues and Westman and colleagues are suggested, and direct observation of the specific binding of ribavirin to eIF4E by using mass spectrometry is presented.