Auxin response factor family in rice.
Auxin response factor family in rice.
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DOI：
10.1266/ggs.76.373
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发表时间：
2001-12
影响因子：
1.1
通讯作者：
Y. Sato;A. Nishimura;M. Ito;M. Ashikari;H. Hirano;M. Matsuoka
中科院分区：
文献类型：
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作者：
Y. Sato;A. Nishimura;M. Ito;M. Ashikari;H. Hirano;M. Matsuoka
我们分离了11个与拟南芥生长素反应因子（ARF）基因同源的水稻基因。所有的基因编码一个高度保守的氨基酸序列的N-末端区域，这被认为是一个DNA结合域（DBD）。系统发育分析的基础上比较的DBDs表明，水稻有一个或两个密切相关的直系同源物对应于一个给定的各自的ARF基因在拟南芥。我们还分析了C-末端保守结构域（CTD）中的另一个保守结构域的氨基酸序列，该保守结构域是除OsETTIN 1和OsETTIN 2外几乎所有水稻ARF所共有的。这些结果与从DBD比较中推导出的进化关系一致。与许多ARF相反，OsETTIN 1和OsETTIN 2不包含保守的C-末端结构域，但确实共享另一个在拟南芥ETTIN中也发现的共有基序。所有上述观察结果表明，水稻具有功能多样的ARF基因，其结构和功能对应于各种拟南芥ARF的结构和功能，一个或两个水稻ARF对应于给定的拟南芥ARF。因此，生长素信号转导机制可能在单子叶植物和双子叶植物之间很好地保守。
We isolated 11 rice genes homologous to the genes encoding auxin response factors (ARFs) in Arabidopsis. All of the genes encoded a well-conserved amino acid sequence in the N-terminal region, which is considered to be a DNA-binding domain (DBD). Phylogenetic analysis based on comparison of the DBDs indicated that rice has one or two closely related orthologs corresponding to a given respective ARF gene in Arabidopsis. We also analyzed the amino acid sequences of another conserved domain in the C-terminal conserved domain (CTD), which was shared by almost all the rice ARFs, with the exception of OsETTIN1 and OsETTIN2. These results agreed well with the evolutionary relationship deduced from the DBD comparison. In contrast to many ARFs, OsETTIN1 and OsETTIN2 do not contain the conserved C-terminal domain, but do share another consensus motif that is also found in Arabidopsis ETTIN. All of the above observations indicate that rice has functionally diversified ARF genes whose structures and functions correspond to those of various Arabidopsis ARFs, with one or two rice ARFs corresponding to a given Arabidopsis ARF. Thus, auxin signal transduction mechanisms may be well conserved between monocot and dicot plants.