Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells
Effects of zinc on the mineralization of bone nodules from human osteoblast-like cells
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DOI：
10.1007/bf02685919
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发表时间：
2007-04-01
影响因子：
3.9
通讯作者：
El-Sohemy, Ahmed
中科院分区：
文献类型：
--
作者：
Cerovic, Aleksandra;Miletic, Ivanka;El-Sohemy, Ahmed
锌是骨骼正常发育所需的重要矿物质。然而，锌对人骨细胞矿化的直接影响尚不清楚。本研究的目的是确定锌对SaOS-2人成骨样细胞的分化和矿化骨结节形成的影响。将细胞培养8天，然后转移到无锌培养基中并用不同浓度（0-50 μ M）的锌处理。碱性磷酸酶（ALP）活性作为成骨细胞分化的指标，vonKossa染色检测骨结节。在处理4、6和8 d后，锌在1和10 μ M时增加ALP活性，但在50 μ M时降低活性。经过9天的治疗，锌增加了矿化骨结节的数量和面积在低浓度（1和10 μ M），但在较高浓度（25和50 μ M）下降。这些结果表明，锌对人成骨样细胞的分化和矿化具有双相作用。
Zinc is an important mineral that is required for normal bone development. However, the direct effects of zinc on the mineralization of bone cells of human origin are not clear. The objective of this study was to determine the effects of zinc on the differentiation of SaOS-2 human osteoblast-like cells and the formation of mineralized bone nodules. Cells were cultured for 8 d and then transferred to zinc-free medium and treated with varying concentrations (0-50 mu M) of zinc. Alkaline phosphatase (ALP) activity was used as a measure of osteoblast differentiation, and bone nodules were detected by von Kossa staining. After 4, 6, and 8 d of treatment, zinc increased ALP activity at 1 and 10 mu M, but decreased activity at 50 mu M. After 9 d of treatment, zinc increased both the number and area of mineralized bone nodules at low concentrations (1 and 10 mu M), but decreased both at higher concentrations (25 and 50 mu M). These findings demonstrate that zinc has biphasic effects on the differentiation and mineralization of human osteoblast-like cells.