CHARACTERIZATION OF FACTOR-XIIIA POSITIVE DERMAL DENDRITIC CELLS IN NORMAL AND INFLAMED SKIN
CHARACTERIZATION OF FACTOR-XIIIA POSITIVE DERMAL DENDRITIC CELLS IN NORMAL AND INFLAMED SKIN
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DOI：
10.1111/j.1365-2133.1989.tb15509.x
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发表时间：
1989-10-01
影响因子：
10.3
通讯作者：
HEADINGTON, JT
中科院分区：
文献类型：
--
作者：
CERIO, R;GRIFFITHS, CEM;HEADINGTON, JT
使用针对凝血酶因子 XIII 亚基 A (FXIIIa) 的兔多克隆抗体对正常和发炎的人体皮肤组织进行免疫细胞化学鉴定和表征本土真皮树突状细胞（真皮树突状细胞）。使用免疫过氧化物酶和双免疫荧光染色技术，用一组 18 种单克隆抗体分析 FXIIIa 阳性树突状细胞的免疫表型。针对 FXIIIa 的抗体检测到高度树突状真皮细胞，特别是位于上层网状和乳头状真皮。双荧光显微镜显示 FXIIIa 阳性细胞来自骨髓 (HLe-I+)，并共表达单核细胞、巨噬细胞或抗原呈递细胞标记物 (HLA-DR+、LFA-I+、HLA-DQ+、OKM5+、MoI+、Mono-I+、Leu M3+)。朗格汉斯细胞 (CDI)、T 淋巴细胞 (CD2)、粒细胞 (LeuMI)、成纤维细胞 (Te7)、细胞间粘附分子-I (ICAM-I) 或内皮细胞（因子 VIII 相关抗原）的细胞标记物未获得标记。γ 干扰素诱导 HLA-DR 表达增加和 器官培养中正常皮肤中 FXIIIa+ 真皮树突细胞上的 ICAM-I。此外，在特应性湿疹和银屑病等良性炎症性皮肤病中，真皮上层中的 FXIIIa+、DR+、ICAM-I+ 细胞数量增加，表皮中的 FXIIIa+ 细胞灶与淋巴细胞密切相关。人类皮肤中的 FXIIIa 阳性细胞代表骨髓真皮树突状细胞的特定群体，不同于 朗格汉斯细胞，具有单核吞噬细胞（单核细胞/巨噬细胞）的一些共同特征。此外，在 48% 的 FXIIIa+ 细胞上检测到 HLA-DQ，并且缺乏 OKMI 与高 OKM5 表达相结合，表明存在抗原呈递细胞表型。
The immunocytochemical identification and characterization of indigenous dermal dendritic cells (dermal dendrocytes) using a rabbit polyclonal antibody to clotting enzyme factor XIII subunit A (FXIIIa) was carried out on normal and inflamed human cutaneous tissue. The immunophenotype of FXIIIa positive dendritic cells was analysed with a panel of 18 monoclonal antibodies using immunoperoxidase and double immunofluorescence staining techniques.The antibody against FXIIIa detected highly dendritic dermal cells located particularly in the upper reticular and papillary dermis. Double fluorescence microscopy showed that FXIIIa positive cells were bone marrow derived (HLe‐I+) and co‐expressed monocyte, macrophage or antigen presenting cell markers (HLA‐DR+, LFA‐I+, HLA‐DQ+, OKM5+, MoI+, Mono‐I+, Leu M3+). No labelling was obtained with cell markers for Langerhans cells (CDI), T lymphocytes (CD2), granulocytes (LeuMI) fibroblasts (Te7), intercellular adhesion molecule‐I (ICAM‐I) or endothelial cells (Factor VIII related antigen).Gamma interferon induced increased expression of HLA‐DR and co‐expression of ICAM‐I on FXIIIa+dermal dendritic cells in normal skin in organ culture. Moreover, in benign inflammatory dermatoses such as atopic eczema and psoriasis there was an increased number of FXIIIa+, DR+, ICAM‐I+cells in the upper dermis and foci of FXIIIa+cells in the epidermis closely associated with lymphocytes.FXIIIa positive cells in human skin represent a specific population of bone‐marrow dermal dendritic cells, distinct from Langerhans cells, that share some features common to mononuclear phagocytes (monocyte/macrophages). In addition, the detection of HLA‐DQ on 48% of FXIIIa+cells and the lack of OKMI in combination with high OKM5 expression suggests an antigen‐presenting cell phenotype.