TISSUE GLUCOSE-UTILIZATION DURING EPINEPHRINE-INDUCED HYPERGLYCEMIA
TISSUE GLUCOSE-UTILIZATION DURING EPINEPHRINE-INDUCED HYPERGLYCEMIA
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DOI：
10.1152/jappl.1989.67.5.1770
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发表时间：
1989-11-01
影响因子：
3.3
通讯作者：
SPITZER, JJ
中科院分区：
文献类型：
--
作者：
MESZAROS, K;LANG, CH;SPITZER, JJ
本研究的目的是研究肾上腺素输注过程中单个组织的葡萄糖利用。首先，2-脱氧葡萄糖（2-DG）示踪技术在体内高血糖症的适用性进行了研究，在体外模型系统。用1.25-20 mM葡萄糖孵育滑车上膜肌和脾细胞。对2-[14 C]DG在葡萄糖代谢途径的歧视，表示的集总常数，保持不变，在这个广泛的葡萄糖浓度范围。得出的结论是，体内高血糖症并不排除2-DG方法的应用。在一系列体内实验中，长期插管的清醒大鼠禁食24小时，并输注肾上腺素（0.2 μ g cntdot）。kg-1. min-1），其产生血浆葡萄糖浓度的两倍增加。在开始肾上腺素输注后30分钟注射2-[14 C]DG，并根据70分钟时切除的样品中磷酸化2-DG的浓度计算单个组织的葡萄糖利用率。肾上腺素输注使后肢肌肉、皮肤、回肠、肝、脾、肺、脂肪和肾脏的葡萄糖利用率增加40-160%，尽管大脑中没有发现变化。血浆葡萄糖增加的质量作用可能在葡萄糖利用率提高中起重要作用。
The aim of this study was to investigate glucose utilization by individual tissues during epinephrine infusion. First, the applicability of the 2-deoxyglucose (2-DG) tracer technique during in vivo hyperglycemia was investigated in model systems in vitro. Epitrochlearis muscle and spleen cells were incubated with 1.25-20 mM glucose. The discrimination against 2-[14C]DG in glucose metabolic pathways, expressed by the lumped constant, remained unchanged over this wide range of glucose concentrations. It was concluded that in vivo hyperglycemia does not preclude the application of the 2-DG method. In a series of in vivo experiments, chronically catheterized conscious rats fasted for 24 h and were infused with epinephrine (0.2 .mu.g .cntdot. kg-1 .cntdot. min-1), which produced a two-fold increase in plasma glucose concentration. 2-[14C]DG was injected 30 min after starting the epinephrine infusion and glucose utilization rates of individual tissues were calculated based on the concentration of phosphorylated 2-DG in samples excised at 70 min. The epinephrine infusion increased glucose utilization rates by 40-160% in hindlimb muscles, skin, ileum, liver, spleen, lung, epidydimal fat, and kidney, although no change was found in the brain. Mass action of the increased plasma glucose is likely to play an important role in the enhanced rate of glucose utilization.