Clonal Spread of Extended-Spectrum Cephalosporin-Resistant Enterobacteriaceae Between Companion Animals and Humans in South Korea
Clonal Spread of Extended-Spectrum Cephalosporin-Resistant Enterobacteriaceae Between Companion Animals and Humans in South Korea
复制标题
DOI：
10.3389/fmicb.2019.01371
复制
发表时间：
2019-06-18
影响因子：
5.2
通讯作者：
Jeong, Seok Hoon
中科院分区：
文献类型：
--
作者：
Hong, Jun Sung;Song, Wonkeun;Jeong, Seok Hoon
超广谱头孢菌素（ESC）耐药肠杆菌科是人类和兽医学中日益重要的问题。本研究的目的是描述携带ESC耐药基因（编码超广谱β-内酰胺酶（ESBL）和AmpC）的肠杆菌科细菌的比较分子特征，这些细菌分离自人粪便样本、伴侣动物直肠拭子和韩国兽医医院环境拭子，并研究其可能的传播和传播。通过PCR和测序鉴定ESC耐药基因。通过脉冲场凝胶电泳（PFGE）和多位点序列分型，对具有优势ESC抗性基因的分离株进行遗传相关性评估。在CHROMagar ESBL上，从人、伴侣动物和兽医医院环境样本中共回收了195株表现出ESC耐药性的大肠埃希菌和41株肺炎克雷伯菌分离株。在伴侣动物中，大多数ESC耐药基因是bla（CMY-2样）（26.4%），其次是bla（CTX-M-55）（17.2%）和bla（CTX-M-14）（16.1%），而bla（CTX-M-15）（28.6%）在人类样本中占主导地位。对携带ESC耐药基因的124株E. coli和23 K.通过PFGE分析携带CMY-2样、DHA-1样或/和CTX-M型的肺炎分离株。对5只E.来自不同兽医诊所的携带bla（CMY-2-like）的大肠杆菌分离株（三个来自狗，两个来自人），其归因于序列类型405，显示出>85%的相似性。我们的研究结果表明，直接传输和传播的ESC耐药肠杆菌科之间的人类和伴侣动物。
Extended-spectrum cephalosporin (ESC)-resistant Enterobacteriaceae is an increasingly important problem in both human and veterinary medicine. The aims of this study were to describe a comparative molecular characterization of Enterobacteriaceae carrying ESC resistance genes, encoding extended-spectrum beta-lactamase (ESBL) and AmpC, isolated from human stool samples, rectal swabs from companion animals, and swabs from the environment of veterinarian hospitals in South Korea, and to examine their possible dissemination and transmission. The ESC resistance genes were identified by PCR and sequencing. Isolates with the predominant ESC resistance genes were assessed for their genetic relatedness by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing. A total of 195 Escherichia coli and 41 Klebsiella pneumoniae isolates that exhibited ESC resistance were recovered on CHROMagar ESBL from human, companion animal, and the veterinary hospital environmental samples. In companion animals, most of the ESC resistance genes were bla(CMY-2-like) (26.4%), followed by bla(CTX-M-55) (17.2%) and bla(CTX-M-14) (16.1%), whereas bla(CTX-M-15) (28.6%) was predominant in human samples. The epidemiological relatedness of isolates carrying ESC resistance genes, including 124 E. coli and 23 K. pneumoniae isolates carrying CMY-2-like, DHA-1-like, or/and CTX-M-type, were analyzed by PFGE. The pulsotypes of five E. coli isolates (three from dogs and two from humans) carrying bla(CMY-2-like), which were attributed to sequence type 405, from different veterinary clinics showed >85% similarity. Our results indicate direct transmission and dissemination of ESC-resistant Enterobacteriaceae between humans and companion animals.