Secretion of tissue-type plasminogen activator and plasminogen activator inhibitor by cultured human endothelial cells: modulation by thrombin, endotoxin, and histamine.
Secretion of tissue-type plasminogen activator and plasminogen activator inhibitor by cultured human endothelial cells: modulation by thrombin, endotoxin, and histamine.
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培养的人内皮细胞分泌组织型纤溶酶原激活剂和纤溶酶原激活剂抑制剂：凝血酶、内毒素和组胺的调节。
DOI：
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
D. Collen
中科院分区：
文献类型：
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作者：
M. Hanss;D. Collen
取4~6个人脐静脉内皮细胞进行原代培养，在含20%人血清的M199细胞培养液中进行融合培养。将融合培养物与无血清培养液(1.5ml/25cm2细胞表面)孵育24小时后，检测条件培养液中组织型纤溶酶原激活剂抗原(t-PA Ag)的分泌及快速作用的纤溶酶原激活物抑制物活性(PA-I活性)。T-PA Ag的基础产量为2.7+/-1.4 ng/ml(Mean+/-SD)，PA-I活性为36+/-18IU/ml。凝血酶刺激后，这两种成分的分泌均呈剂量和时间依赖性增加。在每毫升1NIH U凝血酶时，t-PA Ag增加4倍，PA-I活性增加2倍。这种作用依赖于凝血酶中的一个游离活性部位和特定的蛋白质合成(被放线菌素和放线菌素抑制)，但与前列环素的合成无关(阿司匹林或消炎痛不起作用)。无论是刺激培养的内皮细胞还是非刺激培养的内皮细胞，在条件培养液中的纤维蛋白平板上均未显示出游离t-PA活性。内毒素刺激可引起PA-I活性的特异性分泌，而t-PA-Ag无明显变化。组胺刺激浓度为0.1~1mumol/L时，可引起t-PA Ag的特异性分泌，但不能引起PA-I的特异性分泌。这些发现表明，t-PA抗原的分泌和PA-I活性的分泌并不一定是偶联的。
Primary cultures of pooled endothelial cells obtained from four to six human umbilical cord veins were grown to confluency in M 199 cell culture medium containing 20% human serum. The secretion of tissue-type plasminogen activator antigen (t-PA Ag) and fast-acting plasminogen activator inhibitor activity (PA-I activity) was measured in the conditioned medium after incubation of confluent cultures with serum-free medium for 24 hours (1.5 ml/25 cm2 cell surface). The baseline production of t-PA Ag was 2.7 +/- 1.4 ng/ml (mean +/- SD) and of PA-I activity 36 +/- 18 IU/ml. Stimulation with thrombin resulted in a dose-dependent and time-dependent increase of the secretion of both components. At 1 NIH U thrombin per milliliter, a fourfold increase of t-PA Ag and a twofold increase of PA-I activity were observed. This effect was dependent on a free active site in thrombin and specific protein synthesis (inhibited by cycloheximide and dactinomycin) but unrelated to prostacyclin synthesis (no effect of aspirin or indomethacin). No free t-PA activity could be demonstrated on fibrin plates in conditioned medium from either stimulated or nonstimulated endothelial cell cultures. Endotoxin stimulation resulted in a specific secretion of PA-I activity but not of t-PA Ag, whereas histamine stimulation at concentrations of 0.1 to 1 mumol/L caused a specific secretion of t-PA Ag but not of PA-I. These findings indicate that the secretion of t-PA Ag and PA-I activity are not necessarily coupled.(ABSTRACT TRUNCATED AT 250 WORDS)
DOI：
10.1016/s0021-9258(17)42691-3
发表时间：
1984-12
期刊：
The Journal of biological chemistry
影响因子：
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作者：
J. V. van Mourik;D. Lawrence;D. Loskutoff
通讯作者：
J. V. van Mourik;D. Lawrence;D. Loskutoff
凝血酶介导的人脐静脉内皮细胞培养物中因子 VIII 抗原的释放。
影响因子：
20.3
作者：
Levine,JD;Harlan,JM;Harker,LA;Joseph,ML;Counts,RB
通讯作者：
Counts,RB
DOI：
10.1073/pnas.78.4.2249
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
ESMON, CT;OWEN, WG
通讯作者：
OWEN, WG