Whole genome deoxyribonucleic acid microarray analysis of gene expression in ectopic versus eutopic endometrium
Whole genome deoxyribonucleic acid microarray analysis of gene expression in ectopic versus eutopic endometrium
复制标题
DOI：
10.1016/j.fertnstert.2007.01.056
复制
发表时间：
2007-12-01
影响因子：
6.7
通讯作者：
Hansen, Keith A.
中科院分区：
文献类型：
--
作者：
Eyster, Kathleen M.;Klinkova, Olga;Hansen, Keith A.
目的：使用DNA微阵列鉴定在位子宫内膜与异位子宫内膜中差异表达的基因。设计：前瞻性、横断面、观察性研究。设置：大学医学中心和研究实验室。患者：11名子宫内膜异位症妇女。干预：无。主要结果测量：差异基因表达。结果：在整个人类DNA微阵列上的53，000个探针中，有717个在异位子宫内膜与在位子宫内膜中发生了两倍或更多的变化。差异表达的基因家族包括编码与免疫系统和炎症途径相关的蛋白质、细胞粘附、细胞-细胞连接、细胞外基质及其重塑、细胞骨架蛋白和信号转导途径组分等的基因。结论（S）：改变的免疫环境可能使进入腹腔的异位细胞存活。细胞粘附相关基因的改变可能有助于异位内膜的粘附和侵袭特性，并且信号转导通路的改变支持与在位内膜相比子宫内膜外植体细胞之间的通信的改变。这些差异表达基因的扇形百合花为发展和检验关于子宫内膜异位症的新假说提供了多种机会。（Fertil Steril（（R））2007;88：1505-33. (c)2007年，美国生殖医学会（American Society for Reproductive Medicine）
Objective: To use DNA microarrays to identify differentially expressed genes in eutopic endometrium compared with ectopic endometrium.Design: Prospective, cross-sectional, observational study.Setting: University Medical Center and Research Laboratory.Patient(s): Eleven women with endometriosis.Intervention(S): None.Main Outcome Measure(s): Differential gene expression.Result(s): Seven hundred seventeen of the 53,000 probes on the whole human DNA microarrays were changed by twofold or greater in ectopic versus eutopic endometrium. Families of genes that were expressed differentially include genes that code for proteins associated with the immune system and inflammatory pathways, cell adhesion, cell-cell junctions, the extracellular matrix and its remodeling, cytoskeletal proteins, and signal transduction pathway components, among others.Conclusion(S): The altered immune environment may allow survival of endometriotic cells that enter the abdominal cavity. Alterations of cell adhesion-associated genes may contribute to the adhesive and invasive properties of ectopic endometrium, and changes in signal transduction pathways support a change in the communication among cells of the endometrial explant compared with eutopic endometrium. These fan-Lilies of differentially expressed genes provide multiple opportunities for the development and testing of new hypotheses regarding endometriosis. (Fertil Steril((R)) 2007;88:1505-33. (c) 2007 by American Society for Reproductive Medicine.)