A novel repeated sequence located on the bovine Y chromosome: Its application to rapid and precise embryo sexing by PCR
A novel repeated sequence located on the bovine Y chromosome: Its application to rapid and precise embryo sexing by PCR
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DOI：
10.1292/jvms.66.509
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发表时间：
2004-05-01
影响因子：
1.2
通讯作者：
Chikuni, K
中科院分区：
文献类型：
--
作者：
Kageyama, S;Yoshida, I;Chikuni, K
发现了一个新的雄性牛特异性重复序列，命名为S4。S4是高度重复的序列，并且是包含各种内部重复序列的1.5kb重复单元。FISH分析表明，S4定位于整个Y染色体长臂和短臂近端区域。我们发现，一个PCR引物集S4扩增男性特异性的178 bp的产物，除了一个145 bp的产物共同的男性和女性细胞。虽然145 bp产物的来源是未知的，它作为一个积极的内部控制在实际的胚胎性别鉴定。由于S4的高拷贝数，PCR仅需要0.5pg纯化的DNA就可以准确扩增。这使得有可能减少胚胎性别鉴定所需的活检样本量，从而减少对操作胚胎的损伤。这些研究表明，基于S4序列的胚胎性别鉴定是准确和敏感的。
A novel repeated sequence specific to male cattle was identified and named S4. S4 is a highly repetitive sequence and is a 1.5 kb repeating unit that contains various internal repeated sequences. FISH analysis showed that S4 is localized on the whole long arm and the proximal region of the short arm of the Y chromosome. We found that a PCR primer set for S4 amplified a male-specific 178 bp product in addition to a 145 bp product common to both male and female cells. Although the origin of the 145 bp product is unknown, it acts as a positive internal control in practical embryo sexing. Due to the high copy number of S4, PCR required only 0.5 pg purified DNA for accurate amplification. This made it possible to reduce the amount of biopsy sample required for embryo sexing and thus result in less damage to embryos manipulated. These studies indicate that embryo sexing based on the S4 sequence is accurate and sensitive.