Behavioral and physiological mouse assays for anxiety: A survey in nine mouse strains.
Behavioral and physiological mouse assays for anxiety: A survey in nine mouse strains.
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DOI：
10.1016/s0166-4328(02)00200-0
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发表时间：
2002-11-15
影响因子：
2.7
通讯作者：
Paylor, Richard
中科院分区：
文献类型：
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作者：
Bouwknecht, J. Adriaan;Paylor, Richard
为了找到更好的和新的治疗人类焦虑的方法，各种范式被用来研究啮齿动物的焦虑相关过程。我们研究了小鼠在两个不同的焦虑相关的测定：生理应激诱导的高热（SIH）范式和行为光暗探索（LD）测试。在两种试验中重复检测了8个近交系（129 S6/SvEvTac、129 S1/SvImJ、A/J、BALB/cByJ、C3 H/HeJ、C57 BL/6 J、DBA/2 J和FVB/NJ）和1个远交系（CD 1-ICR）。本研究描述了第一个应变调查的SIH范式。所有菌株均显示出SIH反应，但反应的幅度在品系之间变化。LD试验中行为反应的近交系分布模式与SIH反应不相关。缺乏显着的相关性表明，这些反应之间没有遗传关系。小鼠可以在两种测定中重复测试而不影响结果。一个新的范例，在这两种检测相结合，阐明了行为反应没有改变的SIH范例的部分。相比之下，暴露于光暗箱而不是家庭笼中对生理反应表现出应变依赖性影响。我们的结论是，行为和生理反应的结合可能会导致更好地了解焦虑相关的过程。
In order to find better and new treatments for anxiety in humans, a variety of paradigms are used to study anxiety-related processes in rodents. We studied mice in two different anxiety-related assays: the physiological stress-induced hyperthermia (SIH) paradigm and the behavioral light-dark exploration (LD) test. Eight inbred strains (129S6/SvEvTac, 129S1/SvImJ, A/J, BALB/cByJ, C3H/HeJ, C57BL/6J, DBA/2J, and FVB/NJ) and one outbred strain (CD1-ICR) were tested in both assays repeatedly. This study describes the first strain survey for the SIH paradigm. All strains showed an SIH response, but the magnitude of the response varied between lines. The inbred strain distribution pattern for the behavioral responses in the LD assay was not correlated with the SIH response. The lack of a significant correlation suggests that there is no genetic relation between such responses. Mice could be tested repeatedly in both assays without affecting the results. A new paradigm, in which both assays were combined, elucidated that behavioral responses were not altered by segments of the SIH paradigm. In contrast, exposure to the light-dark box instead of the home-cage showed a strain-dependent effect on the physiological response. We conclude that a combination of behavioral and physiological responses might lead to a better understanding in anxiety-related processes.