SOLID-PHASE RADIOIMMUNOASSAYS FOR HUMAN C-REACTIVE PROTEIN
SOLID-PHASE RADIOIMMUNOASSAYS FOR HUMAN C-REACTIVE PROTEIN
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DOI：
10.1016/0009-8981(81)90005-x
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发表时间：
1981-01-01
影响因子：
5
通讯作者：
PEPYS, MB
中科院分区：
文献类型：
--
作者：
SHINE, B;DEBEER, FC;PEPYS, MB
利用与可磁化纤维素颗粒偶联的抗血清建立了两种新的、快速且灵敏的人C -反应蛋白（CRP）放射免疫测定法，这种偶联便于相分离。一种单抗体方法，使用固相抗 - CRP，在1小时孵育时间下灵敏度为50微克/升，测定内和测定间变异系数为10%。一种双抗体方法，使用液相兔抗 - CRP血清和固相羊抗兔IgG血清，在过夜孵育下灵敏度为3微克/升，测定内和测定间变异系数为10%。在来自正常成年志愿献血者的468份血清中，CRP浓度中位数为800微克/升，四分位间距为340 - 1700微克/升，范围为70 - 29000微克/升。90%的样本含量低于3毫克/升，99%低于10毫克/升。在羊水和脑脊液中均检测到低水平（14 - 650微克/升）的CRP。
Two new, rapid and sensitive radioimmunoassays for human C-reactive protein (CRP) were established using antiserum coupled to magnetizable cellulose particles, which facilitate phase separation. A single antibody method, using solid phase anti-CRP, provides a sensitivity of 50 .mu.g/l with a 1-h incubation time and intra- and inter-assay coefficients of variation of 10%. A double antibody method, using fluid phase rabbit anti-CRP serum and solid phase sheep anti-rabbit IgG serum, provides a sensitivity of 3 .mu.g/l with an overnight incubation and intra- and inter-assay coefficients of variation of 10%. Among 468 sera from normal adult volunteer blood donors the median CRP concentration was 800 .mu.g/l, interquartile range 340-1700 .mu.g/l and range 70-29,000 .mu.g/l. Of samples, 90% contained less than 3 mg/l and 99% less than 10 mg/l. Low levels (14-650 .mu.g/l) of CRP were detected both in amniotic fluids and in CSF.