Peptide mimicry of the meningococcal group C capsular polysaccharide.
Peptide mimicry of the meningococcal group C capsular polysaccharide.
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C 组脑膜炎球菌荚膜多糖的肽模拟。
DOI：
10.1073/pnas.92.9.4021
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发表时间：
1995
影响因子：
11.1
通讯作者：
Kieber-Emmons,T
中科院分区：
文献类型：
--
作者：
Westerink,MA;Giardina,PC;Apicella,MA;Kieber-Emmons,T
对模拟C群脑膜炎球菌荚膜多糖（MCP）的抗独特型抗体6 F9的重链和轻链可变区进行序列分析。6 F9上负责诱导抗MCP抗体应答的免疫原性位点通过这些数据的序列和计算机模型分析来确定。互补决定区3（CDR 3）被发现是独特的，因为序列区YRY暴露在表面上。合成跨越CDR 3结构域的合成肽并与蛋白体（脑膜炎球菌B群外膜蛋白）复合。用肽-蛋白体复合物免疫BALB/c小鼠导致显著的抗MCP抗体应答。免疫的小鼠被保护免于感染致死剂量的脑膜炎奈瑟菌血清群C。
Sequence analysis of the variable regions of the heavy and light chains of the anti-idiotypic antibody 6F9, which mimics the meningococcal group C capsular polysaccharide (MCP), was performed. The immunogenic site on 6F9 responsible for inducing an anti-MCP antibody response was determined by means of sequence and computer model analysis of these data. Complementarity-determining region 3 (CDR3) was found to be unique in that the sequence tract YRY was exposed on the surface. A synthetic peptide spanning the CDR3 domain was synthesized and complexed to proteosomes (meningococcal group B outer membrane protein). Immunizations of BALB/c mice with the peptide-proteosome complex resulted in a significant anti-MCP antibody response. Immunized mice were protected against infection with a lethal dose of Neisseria meningitidis serogroup C.