Characterisation of fluoroquinolone-resistant clinical isolates of Streptococcus pyogenes in Barcelona, Spain.
Characterisation of fluoroquinolone-resistant clinical isolates of Streptococcus pyogenes in Barcelona, Spain.
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西班牙巴塞罗那化脓性链球菌耐氟喹诺酮临床分离株的特征。
DOI：
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发表时间：
2005
影响因子：
14.2
通讯作者：
P. Coll
中科院分区：
文献类型：
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作者：
Alba Rivera;M. Rebollo;F. Sánchez;F. Navarro;E. Miró;B. Mirelis;P. Coll
对6株环丙沙星耐药水平较高或较低的化脓性链球菌进行了耐药机制和克隆关系的研究。4株分离株对环丙沙星和左氧氟沙星的敏感性降低，ParC基因发生改变：Ser 80-->Pro（分离株emm3.1）; Ser 79-->Ala（2株emm6.0）; Ser 79-->Phe和Ala 121-->瓦尔（分离株emm12.27）。2株（emm12.26）对环丙沙星、左氧氟沙星及其他喹诺酮类药物耐药。这些分离物在ParC中具有与分离物emm12.27相同的双取代，并且在GyrA中具有另外的取代（Ser 81->Tyr）。耐药模式，emm分型和喹诺酮耐药决定区的测序确定了两个集群，分别包含三个和两个分离株。
Resistance mechanisms and clonal relationships were determined for six Streptococcus pyogenes isolates with low- or high-level ciprofloxacin resistance. Four isolates displayed reduced susceptibility to ciprofloxacin and levofloxacin and had alterations in ParC: Ser80-->Pro (isolate emm3.1); Ser79-->Ala (two isolates emm6.0); and a double substitution Ser79-->Phe and Ala121-->Val (isolate emm12.27). Two isolates (emm12.26) displayed high-level resistance to ciprofloxacin and levofloxacin, as well as to other quinolones. These isolates had the same double substitution in ParC as isolate emm12.27, and an additional substitution (Ser81-->Tyr) in GyrA. Resistance patterns, emm typing and sequencing of the quinolone resistance-determining regions defined two clusters containing three and two isolates, respectively.