Separation of tocopherol isomers using capillary electrochromatography: comparison of monomeric and polymeric C30 stationary phases.
Separation of tocopherol isomers using capillary electrochromatography: comparison of monomeric and polymeric C30 stationary phases.
复制标题
使用毛细管电色谱法分离生育酚异构体：单体和聚合 C30 固定相的比较。
DOI：
10.1021/ac011018a
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发表时间：
2001
影响因子：
7.4
通讯作者：
I. Warner
中科院分区：
文献类型：
--
作者：
C. Henry;C. Fortier;I. Warner
使用不同的固定相烷基链长度（即，C18对C30）和烷基相连接到二氧化硅基载体的模式（即，聚合物相对于单体合成）。较小的烷基链不表现出拆分所有生育酚异构体所需的形状选择性。相反，聚合物和单体C30相都显示出增加的生育酚异构体选择性。改变移动的相的洗脱强度对选择性有显著影响，向甲醇移动的相中少量加入水产生最佳选择性。使用单体结合的C30固定相和甲醇/水移动的相实现生育酚异构体的完全基线分离。使用NIST标准参比物质检查固定相选择性的差异，以确定LC中的柱选择性。结果表明，单体C30实际上具有“中间”相特征（即，高相负载、封端等）。这种新的基于CEC的分离也用于分离维生素E补充剂样品中的生育酚。
The comparison of tocopherol isomer separation achieved using different stationary-phase alkyl chain lengths (i.e., C18 vs C30) and modes of alkyl phase attachment to the silica-based supports (i.e., polymeric vs monomeric synthesis) using capillary electrochromatography (CEC) has been demonstrated. The smaller alkyl chain does not exhibit the shape selectivity needed to resolve all of the tocopherol isomers. Conversely, both polymeric and monomeric C30 phases show increased tocopherol isomer selectivity. Changing the elutropic strength of the mobile phase had dramatic effects on the selectivity, with small additions of water to a methanol mobile phase yielding the best selectivity. The complete baseline separation of the tocopherol isomers was achieved using the monomerically bound C30 stationary phase and a methanol/ water mobile phase. The differences in stationary-phase selectivity were examined using a NIST standard reference material for determining column selectivity in LC. The results indicated that the monomeric C30 actually had "intermediate" phase characteristics (i.e., high phase loading, end capping, etc). This new CEC-based separation was also used to separate the tocopherols in a vitamin E supplement sample.