The ATP-binding protein FgArb1 is essential for penetration, infectious and normal growth of Fusarium graminearum.
The ATP-binding protein FgArb1 is essential for penetration, infectious and normal growth of Fusarium graminearum.
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DOI：
10.1111/nph.15261
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发表时间：
2018-06
期刊：
影响因子：
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通讯作者：
Y. Yin;Zhihui Wang;Danni Cheng;Xiang Chen;Yun Chen;Zhonghua Ma
中科院分区：
文献类型：
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作者：
Y. Yin;Zhihui Wang;Danni Cheng;Xiang Chen;Yun Chen;Zhonghua Ma
三磷酸腺苷结合盒(ABC)转运体主要作用于跨细胞膜转运化合物，在多种生物过程中起着重要作用。然而，它们在禾谷镰刀菌致病过程中的作用还没有得到很好的描述。对60个禾谷镰刀菌ABC蛋白基因进行了功能分析。其中，FgArb1调控正常生长，对致病起重要作用。因此，本研究对FgArb1在致病性中的调控机制进行了分析。FgArb1与丝裂原活化蛋白激酶(MAPK)FgSte7相互作用，并通过调节FgGpmk1(FgSte7的下游激酶)的磷酸化来部分调节植物的渗透。FgArb1突变体显著减少了受伤宿主组织中的感染性生长，这可能是由于其对氧化应激的敏感性增加、细胞壁完整性缺陷和脱氧雪腐镰刀菌烯醇(DON)产生减少所致。FgArb1对有性孢子和无性孢子的产生也很重要，有性孢子和无性孢子是植物侵染的重要繁殖体。此外，FgArb1还通过阻止核糖体小亚基的核输出参与蛋白质生物合成的调节。最后，FgArb1的K28、K65、K341和K525位的乙酰化修饰是其生物学功能所必需的。综上所述，本研究结果为了解ABC转运蛋白在真菌发病机制中的作用提供了新的视角。
ATP-binding cassette (ABC) transporters act mainly to transport compounds across cellular membranes and are important for diverse biological processes. However, their roles in pathogenesis have not been well-characterized in Fusarium graminearum. Sixty F. graminearum ABC protein genes were functionally characterized. Among them, FgArb1 regulates normal growth and importantly is essential for pathogenicity. Thus, the regulatory mechanisms of FgArb1 in pathogenicity were analyzed in this study. FgArb1 interacts with the mitogen-activated protein kinase (MAPK) FgSte7, and partially modulates plant penetration by regulating the phosphorylation of FgGpmk1 (the downstream kinase of FgSte7). The FgArb1 mutant exhibited dramatically reduced infective growth within wounded host tissues, likely resulting from its increased sensitivity to oxidative stresses, defective cell wall integrity and reduced deoxynivalenol (DON) production. FgArb1 also is important for the production of sexual and asexual spores that are important propagules for plant infection. In addition, FgArb1 is involved in the regulation of protein biosynthesis through impeding nuclear export of small ribosomal subunit. Finally, acetylation modification at sites K28, K65, K341 and K525 in FgArb1 is required for its biological functions. Taken together, results of this study provide a novel insight into functions of the ABC transporter in fungal pathogenesis.