Copper-responsive gene expression in the methanotroph Methylosinus trichosporium OB3b.
Copper-responsive gene expression in the methanotroph Methylosinus trichosporium OB3b.
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DOI：
10.1039/c5mt00289c
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发表时间：
2016-09-01
期刊：
影响因子：
--
通讯作者：
Rosenzweig AC
中科院分区：
文献类型：
--
作者：
Kenney GE;Sadek M;Rosenzweig AC
甲烷营养菌使用甲烷单加氧酶（MMO）将甲烷转化为甲醇。在许多菌株中，可以根据铜的可用性产生含铁可溶性（sMMO）或含铜颗粒（pMMO）酶;这种铜开关的机制尚未阐明。甲烷氧化菌铜稳态中的关键参与者是甲烷氧化菌素（Mbn），其是核糖体产生的、对铜具有高亲和力的后修饰的天然产物。Mbn前体肽编码在一个操纵子内，该操纵子包含一系列推定的转运蛋白、调节蛋白和生物合成蛋白，但这些基因参与Mbn相关过程仍不清楚。对发孢甲基弯菌OB 3b和组成型sMMO产生突变体M. Trichosporium OB 3b PP 358的研究表明，Mbn操纵子确实是铜调控的，为基于生物信息学的鉴定提供了实验支持。此外，Mbn操纵子与sMMO操纵子共调节，并且与pMMO操纵子共调节。在Mbn和sMMO操纵子内，调节基因的子集表现出不同的和共享的表达模式，与它们作为内部调节器的拟议功能一致。此外，还对M. Trichosporium OB 3b PP 358突变体的发现为pMMO操纵子附近的基因参与甲烷氧化菌铜稳态提供了新的证据。
Methanotrophic bacteria convert methane to methanol using methane monooxygenase (MMO) enzymes. In many strains, either an iron-containing soluble (sMMO) or a copper-containing particulate (pMMO) enzyme can be produced depending on copper availability; the mechanism of this copper switch has not been elucidated. A key player in methanotroph copper homeostasis is methanobactin (Mbn), a ribosomally produced, post-translationally modified natural product with a high affinity for copper. The Mbn precursor peptide is encoded within an operon that contains a range of putative transporters, regulators, and biosynthetic proteins, but the involvement of these genes in Mbn-related processes remains unclear. Extensive time-dependent qRT-PCR studies of Methylosinus trichosporium OB3b and the constitutive sMMO-producing mutant M. trichosporium OB3b PP358 show that the Mbn operon is indeed copper-regulated, providing experimental support for its bioinformatics-based identification. Moreover, the Mbn operon is co-regulated with the sMMO operon and reciprocally regulated with the pMMO operon. Within the Mbn and sMMO operons, a subset of regulatory genes exhibits a distinct and shared pattern of expression, consistent with their proposed functions as internal regulators. In addition, genome sequencing of the M. trichosporium OB3b PP358 mutant provides new evidence for the involvement of genes adjacent to the pMMO operon in methanotroph copper homeostasis.
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影响因子：
64.8
作者：
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影响因子：
14.8
作者：
Haynes, Chad A.;Gonzalez, Ramon
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DOI：
10.1073/pnas.0636904100
发表时间：
2003-04-01
影响因子：
11.1
作者：
Arnesano, F;Banci, L;Thompsett, AR
通讯作者：
Thompsett, AR