Increase in radiosensitivity to ionizing radiation related to replacement of thymidine in mammalian cells with 5-bromodeoxyuridine.
Increase in radiosensitivity to ionizing radiation related to replacement of thymidine in mammalian cells with 5-bromodeoxyuridine.
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与用 5-溴脱氧尿苷替代哺乳动物细胞中的胸苷相关，增加对电离辐射的放射敏感性。
DOI：
10.2307/3571865
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发表时间：
1965
影响因子：
3.4
通讯作者：
R. Humphrey
中科院分区：
文献类型：
--
作者：
W. Dewey;R. Humphrey
报告表明，细胞对电离辐射的敏感性（通过集落形成的减少来衡量）与掺入脱氧核糖核酸 (DNA) 中的 5-溴脱氧尿苷 (BUdR) 的量有关 (1, 2)。然而，关于辐射引起的染色体畸变与掺入 DNA 的 BUdR 量之间的相关性尚缺乏研究。由于群体中的细胞并非都具有相同量的 BUdR 掺入量，因此测量对辐射损伤进行评分的相同细胞中 BUdR 的掺入量非常重要。通过对用氚化 BUdR (H3BUdR) 处理的细胞进行放射自显影，可以确定中期细胞中的染色体损伤，并根据颗粒数量确定掺入的 BUdR 量。
Reports have indicated that the sensitization of cells to ionizing radiation, as measured by a reduction in colony formation, is related to the amount of 5-bromodeoxyuridine (BUdR) incorporated into the deoxyribonucleic acid (DNA) (1, 2). Studies are lacking, however, on the correlation between radiation-induced chromosomal aberrations and the amount of BUdR incorporated into the DNA. Because cells in the population will not all have the same amount of BUdR incorporated, it is important to measure the incorporation of BUdR in the same cells in which the radiation damage is scored. By making autoradiographs of cells treated with tritiated BUdR (H3BUdR), it was possible to determine in the metaphase cells both the chromosomal damage and, from the number of grains, the amount of BUdR incorporated.