Changes in gaseous concentration of alkylpyrazine analogs affect mouse avoidance behavior
Changes in gaseous concentration of alkylpyrazine analogs affect mouse avoidance behavior
复制标题
烷基吡嗪类似物气体浓度的变化影响小鼠的回避行为
DOI：
10.1093/bbb/zbab178
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Kashiwayanagi M
中科院分区：
文献类型：
--
作者：
Osada K;Miyazono S;Ohata M;Noguchi T;Kashiwayanagi M
我们开发了一种快速，准确的方法，用于定量气相气味使用顶空固相微萃取（HS-SPME）结合GC-MS和使用我们的系统来量化烷基吡嗪类似物在Y-迷宫。在气相中的挥发性化合物的快速提取实现了在Y-迷宫中的几个位置处的气态烷基吡嗪类似物的准确定量分析。我们还使用了一系列的三个SPME纤维来量化浓度随时间的变化。我们进行了行为测试的小鼠在这些烷基吡嗪的反应，并确定了气体浓度的增加率和回避率诱导之间的正相关关系。我们的研究结果表明，Y-迷宫是一个简单而可靠的设备，嗅觉行为研究。HS-SPME快速提取方法可以量化烷基吡嗪的气体浓度如何随时间变化，并且烷基吡嗪浓度的时间依赖性增加与小鼠厌恶行为的诱导相关。
We developed a rapid and accurate method for quantifying gaseous phase odorants using headspace solid-phase microextraction (HS-SPME) in conjunction with GC-MS and used our system to quantify alkylpyrazine analogs in the Y-maze. Rapid extraction of volatile compounds in the vapor phase achieved accurate quantitative analysis of gaseous alkylpyrazine analogs at several locations in the Y-maze. We also used a series of three SPME fibers to quantify changes in the concentration over time. We conducted a behavioral test of mice in response to these alkylpyrazines and identified a positive relationship between the rate of increase in gaseous concentration and the avoidance rate induced. Our results demonstrate that the Y-maze is a simple but reliable apparatus for behavioral studies of olfaction. The HS-SPME fast extraction method can quantify how gaseous concentrations of alkylpyrazines change over time, and the time-dependent increase of alkylpyrazine concentration is correlated with induction of aversive behavior in mice.