Transcriptome-wide analysis of the difference of alternative splicing in susceptible and resistant silkworm strains after BmNPV infection
Transcriptome-wide analysis of the difference of alternative splicing in susceptible and resistant silkworm strains after BmNPV infection
复制标题
BmNPV感染后易感和抗性家蚕品系选择性剪接差异的全转录组分析
DOI：
10.1007/s13205-019-1669-9
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发表时间：
2019-04-01
期刊：
影响因子：
2.8
通讯作者：
Xu, Anying
中科院分区：
文献类型：
--
作者：
Li, Gang;Zhou, Kaiyue;Xu, Anying
利用高通量RNA测序技术，从家蚕核型多角体病毒（BmNPV）感染后的敏感和抗性家蚕品系中鉴定了新的选择性剪接事件。总之，对于来自BmNPV敏感和抗性蚕品系的两个中肠样品产生了12.82Gb干净的RNA-seq数据，并且对于两个脂肪体样品产生了14.78Gb干净的数据。BmHPV敏感蚕株中的选择性剪接事件和亚型数量多于BmHPV抗性蚕株。此外，家蚕抗核多角体病毒品系中存在的选择性剪接基因主要参与核糖体的功能，而敏感品系中存在的选择性剪接基因主要参与DNA解旋酶活性和信号转导等功能。此外，还鉴定了33个表达的SR或SR样蛋白，其中编码SR或SR样蛋白的三个基因（tetratricopeptide repeat protein 14 homolog，ubiquitin carboxyl-terminal hydrolase 32和zinc finger CCCH domain-containing protein 18）在两个家蚕品系间具有较高的选择性剪接事件。本研究表明，BmNPV处理对BmNPV抗性蚕的mRNA转录影响可能小于BmNPV敏感蚕，且两个蚕系间选择性剪接基因的功能存在差异。
Novel alternative splicing events were identified from BmNPV-susceptible and -resistant silkworm strains after BmNPV infection using high-throughput RNA-sequencing strategy. In total, 12.82 Gb clean RNA-seq data were generated for the two midgut samples from BmNPV-susceptible and -resistant silkworm strains, and 14.78 Gb clean data for the two fat body samples. The number of alternative splicing events and isoforms in the BmNPV-susceptible silkworm strain was more than that in the BmNPV-resistant silkworm strain. Furthermore, alternative splicing genes uniquely present in BmNPV-resistant silkworm strain were involved in functions about ribosome, whereas, alternative splicing genes uniquely present in BmNPV-susceptible silkworm strain were implicated in functions like DNA helicase activity and signal transduction. Additionally, 33 expressed SR or SR-like proteins were identified, and three genes encoding SR or SR-like proteins (tetratricopeptide repeat protein 14 homolog, ubiquitin carboxyl-terminal hydrolase 32andzinc finger CCCH domain-containing protein 18) have a higher number of different alternative splicing events between two silkworm strains. The present study suggested BmNPV treatment may have a smaller effect on the mRNA transcription in BmNPV-resistant silkworms than that in BmNPV-susceptible silkworms, and functions of alternative splicing genes are different between the two silkworm strains.