Increased types I and III collagen and transforming growth factor-beta 1 mRNA and protein in hypertrophic burn scar.
Increased types I and III collagen and transforming growth factor-beta 1 mRNA and protein in hypertrophic burn scar.
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DOI：
10.1111/1523-1747.ep12606979
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发表时间：
1995-05
期刊：
影响因子：
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通讯作者：
Kai Zhang;Warren L. Garner;Leslie Cohen;Jorge L. Rodriguez;S.e.m. Phan
中科院分区：
文献类型：
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作者：
Kai Zhang;Warren L. Garner;Leslie Cohen;Jorge L. Rodriguez;S.e.m. Phan
增生性瘢痕是热损伤后异常愈合的结果。增生性瘢痕的特征在于过度的真皮纤维化和瘢痕形成。5例人增生性瘢痕与正常皮肤进行了比较，使用原位杂交定位mRNA的前胶原I型和III型和转化生长因子β 1。免疫组化法检测I型前胶原和TGF-β 1的表达。结果表明，与正常皮肤相比，增生性疤痕中成纤维细胞的I型和III型前胶原mRNA以及I型前胶原蛋白的表达显着增加。在所有增生性瘢痕病例中，大量细胞表达TGF-β 1 mRNA或肽。在对照组织中未检测到TGF-β 1 mRNA或蛋白。这些结果表明增生性瘢痕中成纤维细胞I型和III型胶原mRNA的产生和表达显著增加。这可能是由于通过旁分泌和自分泌途径增加的TGF-β 1产生造成的，正如对这种纤维化细胞因子所描述的那样。
Hypertrophic scar is the result of abnormal healing that often follows thermal injury. Hypertrophic scar is characterized by excessive dermal fibrosis and scarring. Five cases of human hypertrophic scar were compared with normal skin using in situ hybridization to localize mRNAs for procollagen types I and III and transforming growth factor-beta 1. Expression of type I procollagen and TGF-beta 1 were also examined with immunohistochemistry. The results demonstrated a significant increase in the expression of mRNA for types I and III procollagen and type I procollagen protein by fibroblasts in hypertrophic scar compared with normal skin. In all cases of hypertrophic scar, significant numbers of cells expressed TGF-beta 1 mRNA or peptide. Neither TGF-beta 1 mRNA nor protein was detected in control tissues. These results suggest a profound increase in production and expression of types I and III collagen mRNA by the fibroblasts in hypertrophic scar. This may result from increased TGF-beta 1 production, through paracrine and autocrine pathways, as have been described for this fibrogenic cytokine.