ARABIDOPSIS RBCS GENES ARE DIFFERENTIALLY REGULATED BY LIGHT
ARABIDOPSIS RBCS GENES ARE DIFFERENTIALLY REGULATED BY LIGHT
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DOI：
10.1104/pp.101.3.801
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发表时间：
1993-03-01
期刊：
影响因子：
7.4
通讯作者：
KREBBERS, E
中科院分区：
文献类型：
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作者：
DEDONDER, A;RETHY, R;KREBBERS, E
拟南芥核酮糖-1，5-二磷酸羧化酶/加氧酶小亚基（rbcS）基因家族的单个成员受不同品质的光的差异调节。在10-d龄黄化幼苗中，只有3个基因的表达受光敏色素诱导控制。在红光脉冲后约6小时，rbcs mRNA水平达到最大值（比暗水平高3- 5倍），但是基因之间的衰减速率不同。此外，rbcs 2B需要更高的能量密度进行诱导。在发育的早期阶段，rbcS 1A、2B和3B在黑暗中高度表达，并且不能被红光进一步诱导，这表明在整个调控机制中存在发育成分。连续光照实验表明，高辐照响应可能发挥作用，至少有三个四个rbcS基因的诱导。在光敏色素饱和的条件下，rbcS 1A对蓝光脉冲不敏感，而在三个B基因座基因中，至少rbcS 3B似乎对蓝光光感受器有反应。这些结果增加的数据表明，在高等植物中的rbcS基因家族的个别成员可能会受到各种不同的调节机制。
Individual members of the Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcS) gene family are differentially regulated by light of different qualities. In lo-d-old etiolated seedlings, the expression of only three of the four genes is under inductive phytochrome control. rbcs mRNA levels reach a maximum (3- to 5-fold higher than the dark level) about 6 h after a red light pulse, but the rate of decay differs among the genes. Moreover, rbcs 2B requires a higher fluence for induction. At early stages of development, rbcS 1A, 2B, and 3B are highly expressed in the dark and cannot be further induced by red light, indicating a developmental component in the overall regulatory mechanism. Continuous light experiments indicate that high-irradiance responses may play a role in the induction of at least three of the four rbcS genes. Under conditions of phytochrome saturation, rbcS 1A is insensitive to blue light pulses, whereas among the three B locus genes, at least rbcS 3B appears to respond to a blue-light photoreceptor. These results add to the data suggesting that individual members of rbcS gene families in higher plants may be subject to a variety of differing regulatory mechanisms.