Microvascular recruitment is an early insulin effect that regulates skeletal muscle glucose uptake in vivo
Microvascular recruitment is an early insulin effect that regulates skeletal muscle glucose uptake in vivo
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DOI：
10.2337/diabetes.53.6.1418
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发表时间：
2004-06-01
期刊：
影响因子：
7.7
通讯作者：
Barrett, EJ
中科院分区：
文献类型：
--
作者：
Vincent, MA;Clerk, LH;Barrett, EJ
胰岛素增加葡萄糖进入肌肉的处置。此外，在体内，胰岛素可诱导不同的一氧化氮合酶依赖性血管反应，以增加总骨骼肌血流量和招募肌毛细血管（分别通过松弛阻力和末端小动脉）。在目前的研究中，我们比较了血管和代谢反应的时间序列，以30分钟的生理输注胰岛素（3 mU）。min（-1）。kg（-1），正葡萄糖钳夹）或生理盐水。我们使用对比增强超声连续量化微血管体积。胰岛素在5-10分钟内募集微血管（P < 0.05），这先于胰岛素信号通路的激活和肌肉中葡萄糖处置的增加，以及总腿部血流量的变化。此外，一氧化氮合酶的特异性抑制剂L-NAME（N-omega-nitro-L-caseine-methyl ester）阻断了这种早期微血管募集（P < 0.05），并至少部分抑制了早期肌肉葡萄糖摄取的增加（P < 0.05）。我们的结论是，胰岛素迅速招募骨骼肌毛细血管在体内的一氧化氮依赖性行动，和毛细血管募集的增加可能有助于随后的葡萄糖摄取。
Insulin increases glucose disposal into muscle. In addition, in vivo insulin elicits distinct nitric oxide synthase-dependent vascular responses to increase total skeletal muscle blood flow and to recruit muscle capillaries (by relaxing resistance and terminal arterioles, respectively). In the current study, we compared the temporal sequence of vascular and metabolic responses to a 30-min physiological infusion of insulin (3 mU . min(-1) . kg(-1), euglycemic clamp) or saline in rat skeletal muscle in vivo. We used contrast-enhanced ultrasound to continuously quantify microvascular volume. Insulin recruited microvasculature within 5-10 min (P < 0.05), and this preceded both activation of insulin-signaling pathways and increases in glucose disposal in muscle, as well as changes in total leg blood flow. Moreover, L-NAME (N-omega-nitro-L-arginine-methyl ester), a specific inhibitor of nitric oxide synthase, blocked this early microvascular recruitment (P < 0.05) and at least partially inhibited early increases in muscle glucose uptake (P < 0.05). We conclude that insulin rapidly recruits skeletal muscle capillaries in vivo by a nitric oxide-dependent action, and the increase in capillary recruitment may contribute to the subsequent glucose uptake.