Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM).
Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM).
复制标题
DOI：
10.1016/j.bbrep.2016.09.010
复制
发表时间：
2016-12
影响因子：
2.7
通讯作者：
Imamura T
中科院分区：
文献类型：
--
作者：
Yamamoto S;Oshima Y;Saitou T;Watanabe T;Miyake T;Yoshida O;Tokumoto Y;Abe M;Matsuura B;Hiasa Y;Imamura T
非酒精性脂肪性肝炎（NASH）是由脂肪肝引起的常见肝脏疾病。由于NASH与肝组织的纤维化和形态学变化相关，因此需要直接成像技术来对肝组织进行准确分期。为此，在这项研究中，我们利用了两个无标记的光学成像技术，二次谐波产生（SHG）和自发荧光（AF），使用双光子激发显微镜（TPEM）。NASH模型小鼠组织的三维离体成像，随后进行图像处理，显示SHG和AF足以分别定量表征早期阶段的肝包膜和与肝脏疾病进展相关的实质形态。结合两种无标记光学成像技术的肝脏疾病的评价提出。SHG足以定量表征NASH早期阶段的肝包膜。自体荧光可用于评估与肝脏疾病进展相关的实质形态。
Non-alcoholic steatohepatitis (NASH) is a common liver disorder caused by fatty liver. Because NASH is associated with fibrotic and morphological changes in liver tissue, a direct imaging technique is required for accurate staging of liver tissue. For this purpose, in this study we took advantage of two label-free optical imaging techniques, second harmonic generation (SHG) and auto-fluorescence (AF), using two-photon excitation microscopy (TPEM). Three-dimensional ex vivo imaging of tissues from NASH model mice, followed by image processing, revealed that SHG and AF are sufficient to quantitatively characterize the hepatic capsule at an early stage and parenchymal morphologies associated with liver disease progression, respectively. Combination of two label-free optical imaging techniques for the evaluation of liver disease is proposed. SHG is sufficient to quantitatively characterize the hepatic capsule at an early stage of NASH. Auto-fluorescence is useful to evaluate the parenchymal morphologies associated with liver disease progression.