Th2-specific DNase I-hypersensitive sites in the murine IL-13 and IL-4 intergenic region
Th2-specific DNase I-hypersensitive sites in the murine IL-13 and IL-4 intergenic region
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DOI：
10.1093/intimm/10.12.1981
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发表时间：
1998-12-01
影响因子：
4.4
通讯作者：
Arai, K
中科院分区：
文献类型：
--
作者：
Takemoto, N;Koyano-Nakagawa, N;Arai, K
IL-4和IL-13是由T(H)2细胞优先产生的细胞因子，它们的基因位于人和小鼠的5号染色体和11号染色体上。为了确定IL-4和IL-13基因的T(H)2特异性表达的潜在调控元件，我们构建了IL-13/IL-4基因的物理图谱，并利用T-h克隆和从TCR转基因小鼠获得的体外分化的效应T-h细胞进行了DNase I超敏(DH)位点分析。在IL-13和IL-4基因间隔区有3个水解酶切点：HSS1、HSS2和HSS3。HSS3在T(H)1和T(H)2细胞以及CD4(+)初始T细胞中均可检测到，而HSS1和HSS2仅在T(H)2细胞中检测到。T(H)2亚型分化与HSS1和HSS2出现的相关性提示，这些区域可能在IL-13/IL-4亚型特异性表达中起作用。
IL-4 and IL-13 are cytokines preferentially produced by T(h)2 cells, and their genes are located in close proximity on human chromosome 5 and mouse chromosome 11. To identify potential regulatory elements that confer T(h)2-specific expression of IL-4 and IL-13 genes, we constructed a physical map of the IL-13/IL-4 locus and conducted DNase I-hypersensitive (DH) site analysis using T-h clones and in vitro-differentiated effector T-h cells obtained from TCR transgenic mice. Three DH sites, HSS1, HSS2 and HSS3, were identified within the intergenic region between IL-13 and IL-4 genes. HSS3 was observed both in T(h)1 and T(h)2 cells as well as CD4(+) naive T cells, while HSS1 and HSS2 were detected exclusively in T(h)2 cells. The correlation between differentiation into T(h)2 subtype and the appearance of HSS1 and HSS2 suggests that-these regions may play a role in subtype-specific expression of the IL-13/IL-4 locus.