Antennal transcriptome of Manduca sexta
Antennal transcriptome of Manduca sexta
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DOI：
10.1073/pnas.1017963108
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发表时间：
2011-05-03
影响因子：
11.1
通讯作者：
Hansson, Bill S.
中科院分区：
文献类型：
--
作者：
Grosse-Wilde, Ewald;Kuebler, Linda S.;Hansson, Bill S.
近年来，在了解昆虫嗅觉的分子机制方面取得了相当大的进展。由于所涉及的基因家族的多样性，这一过程在很大程度上依赖于基因组数据。因此，研究集中在具有广泛基因组信息的一小部分物种上。对于鳞翅目这一历史上对嗅觉研究至关重要的大目来说，这种情况主要限制了驯化物种家蚕的进展，基于非公开的部分基因组数据库，夜行动物Heliothis virescens取得了一些进展。由于家蚕的行为能力有限，其生态价值不高，因此研究烟草角虫的分子数据具有重要意义，特别是考虑到其作为经典嗅觉模型的地位及其复杂的自然行为。在这里，我们提出使用转录组学和微阵列数据来识别曼都卡主要嗅觉基因家族的成员。为了评估我们数据的质量，我们将表达受体基因的信息与天线叶的详细形态学数据相关联。最后，我们比较了近完整转录集在雄性和雌性触角中的表达。
In recent years, considerable progress has been made in understanding the molecular mechanisms underlying olfaction in insects. Because of the diverse nature of the gene families involved, this process has largely relied on genomic data. As a consequence, studies have focused on a small subset of species with extensive genomic information. For Lepidoptera, a large order historically crucial to olfactory research, this circumstance has mostly limited advances to the domesticated species Bombyx mori, with some progress in the noctuid Heliothis virescens based on a nonpublic partial genome database. Because of the limited behavioral repertoire and nonexistent ecological importance of Bombyx, molecular data on the tobacco hornworm Manduca sexta are of utmost importance, especially with regards to its position as a classical olfactory model and its complex natural behavior. Here we present the use of transcriptomic and microarray data to identify members of the main olfactory gene families of Manduca. To assess the quality of our data, we correlate information on expressed receptor genes with detailed morphological data on the antennal lobe. Finally, we compare the expression of the near-complete transcript sets in male and female antennae.