THE ESSENTIAL ESCHERICHIA-COLI MSBA GENE, A MULTICOPY SUPPRESSOR OF NULL MUTATIONS IN THE HTRB GENE, IS RELATED TO THE UNIVERSALLY CONSERVED FAMILY OF ATP-DEPENDENT TRANSLOCATORS
THE ESSENTIAL ESCHERICHIA-COLI MSBA GENE, A MULTICOPY SUPPRESSOR OF NULL MUTATIONS IN THE HTRB GENE, IS RELATED TO THE UNIVERSALLY CONSERVED FAMILY OF ATP-DEPENDENT TRANSLOCATORS
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DOI：
10.1111/j.1365-2958.1993.tb01098.x
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发表时间：
1993-01-01
影响因子：
3.6
通讯作者：
GEORGOPOULOS, C
中科院分区：
文献类型：
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作者：
KAROW, M;GEORGOPOULOS, C
我们报告了 msbA 基因的特征，该基因被分离为 HtrB 温度敏感表型的多拷贝抑制基因。 msbA 基因位于大肠杆菌遗传图谱上的 20.5 分钟处，编码一种估计分子量为 64460 Da 的蛋白质，具有完整膜蛋白的特性。 MsbA 的氨基酸序列与 ATP 依赖性易位蛋白家族的氨基酸序列非常相似，其中包括大肠杆菌的溶血素 B 蛋白和哺乳动物多药耐药性 (MDR) 蛋白。 msbA 的突变分析表明，它可能与下游基因 orfE 形成操纵子，并且这两个基因对于所有测试的生长条件下的细菌活力都是必需的。
We report the characterization of the msbA gene, isolated as a multicopy suppressor of the HtrB temperature-sensitive phenotype. The msbA gene maps to 20.5 min on the Escherichia coli genetic map and encodes a protein with an estimated molecular mass of 64460 Da, with the properties of an integral membrane protein. The amino acid sequence of MsbA is very similar to those of the family of ATP-dependent translocators, which includes the haemolysin B protein of E. coli and the mammalian multidrug resistance (MDR) proteins. Mutational analysis of msbA indicates that it may form an operon with a downstream gene, orfE, and that both of these genes are essential for bacterial viability under all growth conditions tested.