Integrated transcriptome and proteome analysis reveals that the antimicrobial griseofulvin targets Didymella segeticola beta-tubulin to control tea leaf spot
Integrated transcriptome and proteome analysis reveals that the antimicrobial griseofulvin targets Didymella segeticola beta-tubulin to control tea leaf spot
复制标题
综合转录组和蛋白质组分析表明，抗菌灰黄霉素靶向 Didymella segeticola β-微管蛋白来控制茶树叶斑病
DOI：
10.1094/phyto-02-22-0061-r
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发表时间：
2022
期刊：
影响因子：
3.2
通讯作者：
Chen Z.
中科院分区：
文献类型：
--
作者：
Huang H. K.;Li D. X.;Jiang S. L.;Yang R.;Yang Y. Q.;Xia Z. Q.;Jiang X. Y.;Zhao Y. T.;Wang. D. L.;Song B. A.;Chen Z.
由于缺乏有效的防治措施，由Didymella segeticol引起的茶斑病在贵州省茶园造成了巨大的损失。筛选对该病原体具有较高控制效果的天然抗菌剂并研究其作用模式可能有助于疾病管理。灰黄青霉衍生的抗菌剂灰黄霉素（GSF）可以抑制D.在离体条件下，半最大有效浓度为0.37 μg/ml，对离体茶枝的治疗效果较好，剂量为25 μg/ml。GSF诱导变形和轻微卷曲的菌丝与扩大的结束，与原生质体凝集在菌丝中，和更多的菌丝突起。GSF改变菌丝形态和亚细胞结构的顺序。整合的转录组和蛋白质组数据显示，在细胞中的物质，细胞运动和有丝分裂的GSF调制的运输。分子对接表明，β-微管蛋白是GSF最有效的靶点，结合自由能为−13.59 kcal/mol，微尺度热泳表明，与β-微管蛋白2相比，GSF与β-微管蛋白1结合的解离常数（Kd）值显著较低。因此，GSF潜在地靶向β-微管蛋白1以干扰染色体分离和真菌有丝分裂，从而抑制菌丝生长。
Because effective control measures are lacking, tea leaf spot caused byDidymella segeticolaresults in huge tea (Camellia sinensis) production losses on tea plantations in Guizhou Province, southwestern China. Screening for natural antimicrobial agents with higher control effects against this pathogen and studying their modes of action may contribute to disease management. Here,Penicillium griseofulvum-derived antimicrobial griseofulvin (GSF) can inhibit the hyphal growth ofD. segeticolastrain GZSQ-4, with a half-maximal effective concentration of 0.37 μg/ml in vitro and a higher curative efficacy at a lower dose of 25 μg/ml for detached tea twigs. GSF induces deformed and slightly curly hyphae with enlarged ends, with protoplasts agglutinated in the hyphae, and higher numbers of hyphal protuberances. GSF alters hyphal morphology and the subcellular structure's order. The integrated transcriptome and proteome data revealed that the transport of materials in cells, cellular movement, and mitosis were modulated by GSF. Molecular docking indicated that beta-tubulin was the most potent target of GSF, with a binding free energy of −13.59 kcal/mol, and microscale thermophoresis indicated that the dissociation constant (Kd) value of GSF binding to beta-tubulin 1, compared with beta-tubulin 2, was significantly lower. Thus, GSF potentially targets beta-tubulin 1 to disturb the chromosomal separation and fungal mitosis, thereby inhibiting hyphal growth.