The HCMV Assembly Compartment Is a Dynamic Golgi-Derived MTOC that Controls Nuclear Rotation and Virus Spread.
The HCMV Assembly Compartment Is a Dynamic Golgi-Derived MTOC that Controls Nuclear Rotation and Virus Spread.
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DOI：
10.1016/j.devcel.2018.03.010
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发表时间：
2018-04-09
影响因子：
11.8
通讯作者：
Walsh D
中科院分区：
文献类型：
--
作者：
Procter DJ;Banerjee A;Nukui M;Kruse K;Gaponenko V;Murphy EA;Komarova Y;Walsh D
Human Cytomegalovirus (HCMV), a leading cause of congenital birth defects, forms an unusual cytoplasmic virion maturation site termed the “Assembly Compartment” (AC). Here, we show that the AC also acts as a microtubule-organizing center (MTOC) wherein centrosome activity is suppressed and Golgi-based MT nucleation is enhanced. This involved viral manipulation of discrete functions of MT plus-end binding (EB) proteins. In particular, EB3, but not EB1 or EB2, was recruited to the AC and was required to nucleate MTs that were rapidly acetylated. EB3-regulated acetylated MTs were necessary for nuclear rotation prior to cell migration, maintenance of AC structure and for optimal virus replication. Independently, a myristoylated peptide that blocked EB3-mediated enrichment of MT regulatory proteins at Golgi regions of the AC also suppressed acetylated MT formation, nuclear rotation and infection. Thus, HCMV offers new insights into the regulation and functions of Golgi-derived MTs, and the therapeutic potential of targeting EB3. Live-cell imaging reveals the dynamic nature of the Human Cytomegalovirus (HCMV) assembly compartment (AC), which acts as a Golgi-derived microtubule-organizing center. Specific recruitment of microtubule plus-end binding EB3 facilitates acetylated MT formation at the AC and nuclear rotation prior to cell migration. EB3 targeting with myristoylated peptides suppresses infection.
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