A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells
A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells
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DOI：
10.1016/j.febslet.2004.05.084
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发表时间：
2004-07-16
期刊：
影响因子：
3.5
通讯作者：
Kanaide, H
中科院分区：
文献类型：
--
作者：
Hirano, K;Hirano, M;Kanaide, H
Rho蛋白对血管内皮细胞S期进程的时间特异性要求通过可逆地引入具有细胞穿透肽的抑制蛋白来确定。我们发现了蛋白质转导可逆性的证据。细胞外蛋白的去除导致转导的蛋白以对低温敏感的方式衰变。50%衰减所需的时间与蛋白质大小相关。抑制蛋白的时间特异性转导揭示了在G（1）-S过渡阶段需要Rho蛋白的关键时期。可逆的蛋白质转导因此可能是一个强大的工具，调查的时间特异性作用的信号蛋白。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The time-specific requirement of Rho proteins for the S phase progression of vascular endothelial cells was determined by reversibly introducing inhibitor proteins with a cell-penetrating peptide. We found evidence of the reversibility of protein transduction. The removal of extracellular protein caused the transduced protein to decay in a manner sensitive to low temperatures. The time required for a 50% decay correlated with the protein size. The time-specific transduction of the inhibitor proteins uncovered a critical period requiring Rho proteins in the G(1)-S transition phase. Reversible protein transduction may thus be a powerful tool to investigate the time-specific role of signaling proteins. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.