HUMAN GIANT-CELL TUMORS OF BONE IDENTIFICATION AND CHARACTERIZATION OF CELL-TYPES
HUMAN GIANT-CELL TUMORS OF BONE IDENTIFICATION AND CHARACTERIZATION OF CELL-TYPES
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DOI：
10.1172/jci112838
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发表时间：
1987-02-01
影响因子：
15.9
通讯作者：
BHAN, AK
中科院分区：
文献类型：
--
作者：
GOLDRING, SR;ROELKE, MS;BHAN, AK
从人骨巨细胞瘤中培养的细胞的特征在于形态特征，增殖能力，存在粒细胞-单核细胞抗原，骨骼激素受体和可溶性细胞产物的基础上。鉴定了三种主要的细胞类型。一个群体由具有成纤维细胞形态的单核细胞组成，其在培养物中增殖并且最可能代表肿瘤的肿瘤成分。从表型上看，它们类似于结缔组织基质细胞。第二个群体的单核细胞缺乏骨骼激素受体，并没有坚持在文化。这些细胞可能是单核细胞-巨噬细胞谱系。第三个细胞群由大型多核巨细胞组成。这些细胞具有破骨细胞的表型特征，包括降钙素受体。人类巨细胞瘤最有可能是结缔组织间质细胞的肿瘤，它有能力招募并与表现出破骨细胞表型特征的多核巨细胞相互作用。
Cells cultured from human giant cell tumors of bone were characterized on the basis of morphological features, proliferative capacity, presence of granulocyte-monocyte antigens, receptors for skeletal hormones, and soluble cell products. Three major cell type were identified. One population consisted of mononuclear cells with fibroblastic morphology, which proliferated in culture and most likely represent the neoplastic element of the tumor. Phenotypically they resembled a connective tissue stromal cell. A second population of mononuclear cells lacked receptors for skeletal hormones and did not persist in culture. These cells were likely of monocyte-macrophage lineage. A third population of cells consisted of large multinucleated giant cells. These cells possessed phenotypic features of osteoclasts including receptors for calcitonin. Human giant cell tumors of none are most likely a neoplasm of connective tissue stromal cells, which have capacity to recruit and interact with multinucleated giant cells that exhibit phenotypic features of osteoclasts.