Measurement of norepinephrine and epinephrine in small volumes of human plasma by a single isotope derivative method: response to the upright posture.
Measurement of norepinephrine and epinephrine in small volumes of human plasma by a single isotope derivative method: response to the upright posture.
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通过单一同位素衍生方法测量少量人血浆中的去甲肾上腺素和肾上腺素：对直立姿势的反应。
DOI：
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发表时间：
1974
影响因子：
5.8
通讯作者：
Suresh D. Shah
中科院分区：
文献类型：
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作者：
Philip E. Cryer;Julio V. Santiago;Suresh D. Shah
摘要：双同位素衍生方法的单同位素改进用于测量去甲肾上腺素和肾上腺素，使用 S-腺苷甲硫氨酸（3H-甲基）而不是 S-腺苷甲硫氨酸（14C-甲基），被发现具有足够的灵敏度（小于 10 pg/ml）和可重复性（去甲肾上腺素和肾上腺素的测定间变异系数为 3.5%）。肾上腺素为 8.5%），用于测量取自仰卧休息受试者的 1.0 ml 人血浆中的儿茶酚胺。在 8 名正常受试者中，平均 (±SE) 血浆去甲肾上腺素浓度从静息仰卧位时的 182 ± 21 pg/ml 升至站立位 5 分钟后的 425 ± 28 pg/ml (p < 0.001)。相应的仰卧位和站立位平均血浆肾上腺素浓度分别为 44 ± 11 pg/ml 和 73 ± 17 pg/ml (p < 0.02)。使用此方法在 18 名受试者中测量的仰卧血浆去甲肾上腺素和肾上腺素浓度与使用母体双同位素方法报告的结果相当。
ABSTRACT A single-isotope modification of the double-isotope derivative method for the measurement of norepinephrine and epinephrine, employing S-adenosylmethionine (3H-methyl) rather than S-andenosylmethionine (14C-methyl), was found to be sufficiently sensitive (to less than 10 pg/ml) and reproducible (interassay coefficient of variation of 3.5% for norepinephrine and 8.5% for epinephrine) for the measurement of these catecholamines in 1.0 ml of human plasma obtained from supine, resting subjects. In 8 normal subjects the mean (±SE) plasma norepinephrine concentration rose from 182 ± 21 pg/ml in the resting, supine position to 425 ± 28 pg/ml (p < 0.001) after 5 mins in the standing position. Corresponding supine and standing mean plasma epinephrine concentrations were 44 ± 11 pg/ml and 73 ± 17 pg/ml (p < 0.02) respectively. Supine plasma norepinephrine and epinephrine concentrations measured in 18 subjects with this method were quite comparable to those reported with the parent double-isotope method.