Chemokine expression in diverse nonimmediate drug hypersensitivity reactions: focus on thymus activation-regulated chemokine, cutaneous T-cell-attracting chemokine, and interleukin-10
Chemokine expression in diverse nonimmediate drug hypersensitivity reactions: focus on thymus activation-regulated chemokine, cutaneous T-cell-attracting chemokine, and interleukin-10
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DOI：
10.1016/j.anai.2014.05.006
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发表时间：
2014-08-01
影响因子：
5.9
通讯作者：
Zhang, Xingqi
中科院分区：
文献类型：
--
作者：
Wang, Fang;He, Dingyang;Zhang, Xingqi
背景：不同类型T淋巴细胞的皮肤浸润与非立即药物超敏反应（niDHRs）的炎症特征有关。吸引皮肤特异性归巢T细胞的重要趋化因子包括胸腺激活调节趋化因子（TARC）和皮肤T细胞吸引趋化因子（CTACK）。白细胞介素-10 （IL-10）是一种有效的趋化因子，可吸引CD8(+) T细胞。目的：探讨niDHRs患者血清TARC、CTACK、IL-10水平及三者之间的相关性。方法：选取Stevens-Johnson综合征合并中毒性表皮坏死松解患者19例、黄斑丘疹样疹患者21例、健康供体21例共40例。获得患者的临床资料。采用酶联免疫吸附法测定血清TARC、CTACK和IL-10水平。结果：nidhr患者血清TARC、CTACK、IL-10水平明显高于正常对照组（P < 0.05、P < 0.001、P < 0.001）。Stevens-Johnson综合征合并中毒性表皮坏死松解患者CTACK和IL-10水平显著高于正常对照组（P < 0.05, P < 0.001）。黄斑丘疹患者TARC、CTACK和IL-10水平高于正常对照组（P < 0.001, P < 0.001, P < 0.05）。40例患者血清CTACK水平与TARC水平均呈正相关（r(s) = 0.3422, P < 0.05）。Stevens-Johnson综合征和中毒性表皮坏死松解患者血清CTACK水平与体表面积脱离呈正相关（r(s) = 0.510, P < 0.05）。结论：这些结果支持了TARC、CTACK和IL-10在niDHRs发病机制中的作用，因为它们具有趋化能力，可以吸引不同的t细胞亚型和不同的niDHRs功能严重程度。(C) 2014年美国过敏、哮喘与免疫学学会。Elsevier Inc.出版。版权所有。
Background: Skin infiltration of different types of T lymphocytes is responsible for inflammatory profiles of nonimmediate drug hypersensitivity reactions (niDHRs). Important chemokines attracting skin-specific homing T cells include thymus activation-regulated chemokine (TARC) and cutaneous T-cell-attracting chemokine (CTACK). Interleukin-10 (IL-10) is a potent chemokine attracting CD8(+) T cells.Objective: To investigate serum levels of TARC, CTACK, and IL-10 in patients with niDHRs and evaluate the correlation among these 3 chemokines.Methods: Forty patients, including 19 patients with Stevens-Johnson syndrome and toxic epidermal necrolysis and 21 patients with maculopapular exanthema, and 21 healthy donors were recruited into the study. Clinical data of patients were obtained. Serum TARC, CTACK, and IL-10 levels were determined by enzyme-linked immunosorbent assay.Results: Serum levels of TARC, CTACK, and IL-10 were significantly elevated in patients with niDHRs compared with those in normal controls (P < .05, P < .001, P < .001, respectively). The CTACK and IL-10 levels were significantly higher (P < .05, P < .001) in patients with Stevens-Johnson syndrome and toxic epidermal necrolysis than in normal controls. Patients with maculopapular exanthema exhibited higher levels of TARC, CTACK, and IL-10 compared with normal controls (P < .001, P < .001, P < .05). Serum CTACK levels were positively correlated with TARC levels in all 40 patients (r(s) = 0.3422, P < .05). Serum CTACK levels positively correlated with detachment of body surface area in patients with Stevens-Johnson syndrome and toxic epidermal necrolysis (r(s) = 0.510, P < .05).Conclusion: These results support a role for TARC, CTACK, and IL-10 in the pathogenesis of niDHRs for their chemotactic ability to attract different T-cell subtypes and different functional severities in niDHRs. (C) 2014 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.