Widespread cytoplasmic mRNA transport in yeast: Identification of 22 bud-localized transcripts using DNA microarray analysis
Widespread cytoplasmic mRNA transport in yeast: Identification of 22 bud-localized transcripts using DNA microarray analysis
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DOI：
10.1073/pnas.2033246100
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发表时间：
2003-09-30
影响因子：
11.1
通讯作者：
Vale, RD
中科院分区：
文献类型：
--
作者：
Shepard, KA;Gerber, AP;Vale, RD
细胞质mRNA定位提供了一种产生细胞不对称和分离蛋白活性的手段。先前的研究已经确定了定位于酿酒酵母芽尖的两种mrna。为了鉴定更多的定位mrna，我们对RNA转运组分She2p、She3p和Myo4p进行了免疫沉淀，并对其相关RNA进行了DNA微阵列分析。二次筛选，使用gfp标记的RNA报告试验，鉴定出22个定位于芽尖的mrna。这些信息编码了多种蛋白质，包括一些参与应激反应和细胞壁维护的蛋白质。许多这些蛋白质不对称地定位在芽上。然而，不对称定位也发生在没有RNA转运的情况下，这表明存在冗余的蛋白质定位机制。与后生动物的发现相反，酵母中的非翻译区域对于mRNA定位是必不可少的。这项研究揭示了一种意想不到的广泛使用RNA运输出芽酵母。
Cytoplasmic mRNA localization provides a means of generating cell asymmetry and segregating protein activity. Previous studies have identified two mRNAs that localize to the bud tips of the yeast Saccharomyces cerevisiae. To identify additional localized mRNAs, we immunoprecipitated the RNA transport components She2p, She3p, and Myo4p and performed DNA microarray analysis of their associated RNAs. A secondary screen, using a GFP-tagged RNA reporter assay, identified 22 mRNAs that are localized to bud tips. These messages encode a wide variety of proteins, including several involved in stress responses and cell wall maintenance. Many of these proteins are asymmetrically localized to buds. However, asymmetric localization also occurs in the absence of RNA transport, suggesting the existence of redundant protein localization mechanisms. In contrast to findings in metazoans, the untranslated regions are dispensable for mRNA localization in yeast. This study reveals an unanticipated widespread use of RNA transport in budding yeast.