Regulation of MAPK function by direct interaction with the mating-specific Gα in yeast
Regulation of MAPK function by direct interaction with the mating-specific Gα in yeast
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DOI：
10.1126/science.1070540
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发表时间：
2002-05-24
期刊：
影响因子：
56.9
通讯作者：
Stone, DE
中科院分区：
文献类型：
--
作者：
Metodiev, MV;Matheos, D;Stone, DE
芽殖酵母酿酒酵母的交配反应是由典型的异源三聚体GTP结合蛋白（G蛋白）和丝裂原活化蛋白激酶（MAPK）级联介导的。虽然这些途径的信号传递已被详细建模，受体后下调是不太好理解。酵母的信息素响应性G蛋白α亚基（Galalpha）下调交配信号，但其靶点尚不清楚。我们已经发现，Galalpha直接结合到酵母细胞响应信息素的交配特异性MAPK。这种相互作用有助于调节交配信号和趋化反应，并且它表明Galpha-MAPK途径的顶部和底部之间的直接通信。
The mating response of the budding yeast Saccharomyces cerevisiae is mediated by a prototypical heterotrimeric GTP-binding protein (G protein) and mitogen-activated protein kinase (MAPK) cascade. Although signal transmission by such pathways has been modeled in detail, postreceptor down-regulation is less well understood. The pheromone-responsive G protein alpha subunit (Galpha) of yeast down-regulates the mating signal, but its targets are unknown. We have found that Galpha binds directly to the mating-specific MAPK in yeast cells responding to pheromone. This interaction contributes both to modulation of the mating signal and to the chemotropic response, and it demonstrates direct communication between the top and bottom of a Galpha-MAPK pathway.