MEMBRANE CHANGES DURING GROWTH OF TETRAHYMENA IN THE PRESENCE OF ETHANOL
MEMBRANE CHANGES DURING GROWTH OF TETRAHYMENA IN THE PRESENCE OF ETHANOL
复制标题
DOI：
10.1016/0005-2736(89)90009-6
复制
发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
THOMPSON, GA
中科院分区：
文献类型：
--
作者：
NANDINIKISHORE, SG;MATTOX, SM;THOMPSON, GA
经过短暂的驯化滞后期，T.梨形菌菌株NT-1能够在含有高水平乙醇的培养基中生长。当在具有1.6%乙醇的培养基中生长时，膜磷脂组成与对照细胞的膜磷脂组成显著不同。主要变化包括十六碳烯酸（16：1和16：2）从23%降至5%，亚油酸（18：2）从14%增至25%。在较低乙醇浓度下生长的细胞中观察到类似但不太明显的变化。乙醇培养的细胞中2-aminoethylphosphonolipid也从脂质磷的16%下降到6%，磷脂酰乙醇胺从39%上升到46%。去除乙醇后，脂质模式迅速恢复正常。为了确定乙醇对膜物理性质的影响，进行了冷冻断裂电子显微镜和荧光偏振研究。正如预期的那样，当乙醇以1.6%存在时，具有明显可检测的流化影响。然而，在含乙醇的培养基中生长引起的膜脂质的变化导致了进一步的流化效果，无论它们是在乙醇的存在或不存在下进行测试。发现的变化是定性相似的，但定量更明显的哺乳动物中观察到的长期暴露于乙醇。
After a brief lag period for acclimation, T. pyriformis strain NT-1 is capable of growing in culture medium containing high levels of ethanol. When growth in a medium having 1.6% ethanol, the membrane phospholipid composition was significantly different from that of control cells. The principal changes included a reduction in hexadecenoic acids (16:1 and 16:2) from 23% to 5% and an increase in linoleic acid (18:2) from 14% to 25%. Similar but less pronounced changes were observed in cells grown in lower ethanol concentrations. There was also a decrease in 2-aminoethylphosphonolipid in the ethanol-grown cells from 16% of the lipid phosphorus to 6% and a coincident rise in the phosphatidylethanolamine from 39% to 46%. The lipid pattern quickly reverted to normal when ethanol was removed. In order to ascertain the effects of ethanol on membrane physical properties, freeze-fracture electron microscopy and fluorescence polarization studies were performed. Ethanol, as expected, had a clearly detectable fluidizing influence when present at 1.6%. However, changes induced in the membrane lipids by growth in the ethanol-containing medium led to a further fluidizing effect, whether they were tested in the presence or absence of ethanol. The alterations found were qualitatively similar but quantitatively more pronounced than those observed in mammals chronically exposed to ethanol.