Methylprednisolone induced neutrophil leukocytosis down modulation of neutrophil L-selectin and Mac-1 expression and induction of granulocyte-colony stimulating factor
Methylprednisolone induced neutrophil leukocytosis down modulation of neutrophil L-selectin and Mac-1 expression and induction of granulocyte-colony stimulating factor
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DOI：
10.1007/s005990050029
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发表时间：
1998-06-01
期刊：
影响因子：
--
通讯作者：
Hawkins, SA
中科院分区：
文献类型：
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作者：
Crockard, AD;Boylan, MT;Hawkins, SA
糖皮质激素诱导的中性粒细胞增多的机制尚未完全清楚;然而，已知白细胞/内皮细胞粘附分子相互作用是中性粒细胞在血管系统内外运动的关键。本研究旨在探讨糖皮质激素对中性粒细胞粘附分子的影响，并与中性粒细胞白细胞增多、循环中性粒细胞计数、中性粒细胞使用和Mac-1表达相关。（通过流式细胞术测量），可溶性L-选择素，粒细胞-在15名接受静脉内甲基强的松龙治疗的多发性硬化症患者中，在最初的500- 600次治疗之前和之后6小时和24小时测定了集落刺激因子浓度。mg剂量。在5天疗程后48 h获得随访样本。中性粒细胞计数在6 h（3倍）和24 h（2倍）升高。这与在6和24 h时L-选择素表达减少40%以及在6 h时Mac-1表达减少35%有关。血清粒细胞集落刺激因子水平增加（6小时：3倍; 24小时：2倍），而可溶性L-选择素浓度不变。在随访样本中，所有上述参数均恢复至基础水平。短期在体外培养（6和24小时）的血液样本未经治疗的多发性硬化症患者和对照组与0.01毫克/毫升甲基强的松龙导致中性粒细胞L-选择素和Mac-1的最小减少和可溶性L-选择素没有变化。粒细胞集落刺激因子诱导Mac-1表达的剂量依赖性的方式，而L-选择素的表达不受影响或减少在高浓度。甲基强的松龙输注后中性粒细胞L-选择素和Mac-1表达减少可能导致边缘中性粒细胞粘附减少和/或中性粒细胞从血管系统迁移的能力降低。此外，粒细胞集落刺激因子的诱导可能有助于中性粒细胞的产生和释放到循环中。
The mechanisms underlying corticosteroid-inducedneutrophil leukocytosis are not fully understood; however, leukocyte/endothelial cell adhesion molecule interactions are known to be key to the movement of neutrophils within and out of the vasculature. This study was designed to investigate the effects of corticosteroids on neutrophil adhesion molecules in relation to neutrophil leukocytosis Circulating neutrophil counts, neutrophil use and Mac-1 expression (measured by flow cytometry), soluble L-selectin, and granulocyte-colony stimulating factor concentrations was determined in 15 multiple sclerosis patients receiving intravenous methylprednisolone prior to and at 6 and 24 h following the initial 500-mg dose. A follow-up sample was obtained 48 h after the 5-day therapeutic course. Neutrophil counts were elevated at 6 h (threefold) and 24 h (twofold). This was associated with a 40% reduction in L-selectin expression at 6 and 24 h and a 35% reduction in Mac-1 expression at 6 h. Serum granulocyte-colony stimulating factor levels were increased (6 h: threefold; 24 h: twofold), whereas soluble L-selectin concentrations were unaltered. All of the above parameters had returned to basal levels in the follow-up sample. Short-term in vitro cultures (6 and 24 h) of blood samples from untreated multiple sclerosis patients and controls with 0.01 mg/ml methylprednisolone resulted in minimal reductions in neutrophil L-selectin and Mac-1 and no change in soluble L-selectin. Granulocyte-colony stimulating factor induced Mac-1 expression in a dose-dependent manner, whereas L-selectin expression was unaffected or reduced at high concentrations. Reduction in neutrophil L-selectin and Mac-1 expression following methylprednisolone infusion may cause decreased adhesion of marginated neutrophils and/or reduced capacity of neutrophils to migrate from the vasculature. Additionally, the induction of granulocyte-colony stimulating factor may contribute to neutrophil production and release into the circulation.