Protection studies in colostrum-deprived piglets of a bovine rotavirus vaccine candidate using human rotavirus strains for challenge.
Protection studies in colostrum-deprived piglets of a bovine rotavirus vaccine candidate using human rotavirus strains for challenge.
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使用人轮状病毒株进行攻击，对剥夺初乳的仔猪进行候选牛轮状病毒疫苗的保护性研究。
DOI：
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发表时间：
1983
影响因子：
6.4
通讯作者：
N. Zygraich
中科院分区：
文献类型：
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作者：
G. Zissis;J. Lambert;P. Marbehant;D. Marissens;M. Lobmann;P. Charlier;A. Delem;N. Zygraich
进行研究以评价牛RIT 4237轮状病毒株作为抗人轮状病毒感染的候选疫苗的潜在用途。初步实验表明，初乳剥夺仔猪容易感染几个人菌株，除了那些属于亚组1。随后，在该动物模型中研究了RIT 4237的不同免疫程序。结果发现，两次给药（肌内给药（两次）或一次肌内给药和一次灌胃给药）对于诱导显著的血清抗体应答是必要的。最后，通过人工攻毒评价了后一种疫苗接种方案对人源轮状病毒亚组2和3株的保护作用。在这两种情况下，与对照动物相比，预先给予活RIT 4237显著降低了攻毒病毒的粪便脱落。
Studies were performed to evaluate the potential use of the bovine RIT 4237 rotavirus strain as a vaccine candidate against infection with human rotaviruses. Initial experiments revealed that colostrum-deprived piglets were susceptible to infection with several human strains, except for those belonging to subgroup 1. Subsequently, different immunization procedures with RIT 4237 were studied in this animal model. It was found that a two-dose administration, either given intramuscularly (twice) or once intramuscularly and once intragastrically, was necessary to induce a significant serum antibody response. Finally, the protective effect of the latter vaccination schedules against subgroup 2 and 3 rotavirus strains of human origin was evaluated by artificial challenge. In both cases, prior administration of live RIT 4237 significantly decreased fecal shedding of the challenge virus when compared with control animals.